Show simple item record

dc.contributor.authorGallego Muñoz, Patricia 
dc.contributor.authorIbares Frías, Lucía
dc.contributor.authorGarrote Adrados, José Antonio 
dc.contributor.authorValsero Blanco, María Cruz
dc.contributor.authorCantalapiedra Rodríguez, Roberto
dc.contributor.authorMerayo Lloves, Jesús
dc.contributor.authorMartínez García, María del Carmen 
dc.date.accessioned2017-01-12T13:06:39Z
dc.date.available2017-01-12T13:06:39Z
dc.date.issued2016
dc.identifier.citationJ Tissue Eng Regen Med. 2016 Nov 15es
dc.identifier.issn1932-6254es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/22096
dc.descriptionProducción Científicaes
dc.description.abstractThe development of treatments that modulate corneal wound healing to avoid fibrosis during tissue repair is important for the restoration of corneal transparency after an injury. To date, few studies have studied the influence of growth factors (GFs) on human corneal fibroblast (HCF) expression of extracellular matrix (ECM) proteins such as collagen types I and III, proteoglycans such as perlecan, or proteins implicated in cellular migration such as α5β1-integrin and syndecan-4. Using in vitro HCFs, we developed a mechanical wound model to study the influence of the GFs basic fibroblast growth factor (bFGF), platelet-derived growth factor (PDGF-BB), and transforming growth factor beta 1 (TGFβ1) on ECM protein production and cellular migration. Our results show that mechanical wounding provokes the autocrine release of bFGF and TGFβ1 at different time points during the wound closure. The HCF response to PDGF-BB was a rapid closure due to fast cellular migration associated with a high focal adhesion replacement and a high expression of collagen and proteoglycans, producing a non-fibrotic healing. bFGF stimulated non-fibrotic ECM production and limited the migration process. Finally, TGFβ1 induced expression of the fibrotic markers collagen type III and α5β1 integrin, and it inhibited cellular migration due to the formation of focal adhesions with a low turnover rate. The novel in vitro HCF mechanical wound model can be used to understand the role played by GFs in human corneal repair. The model can also be used to test the effects of different treatments aimed at improving the healing process.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCórnea - Enfermedadeses
dc.titleHuman corneal fibroblast migration and ECM synthesis during stromal repair: Role played by PDGF-BB, bFGF, and TGFβ1 (HCFs migration and ECM synthesis during stromal repair: GFs effects)es
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1002/term.2360es
dc.identifier.publicationfirstpageppes
dc.identifier.publicationlastpageppes
dc.identifier.publicationtitleJournal of Tissue Engineering and Regenerative Medicinees
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record