The rotational spectrum of the natural amino acid tryptophan has been observed for the first time
using a combination of laser ablation, molecular beams, and Fourier transform microwave spectroscopy.
Independent analysis of the rotational spectra of individual conformers has conducted to
a definitive identification of two different conformers of tryptophan, with one of the observed conformers
never reported before. The analysis of the 14N nuclear quadrupole coupling constants is of
particular significance since it allows discrimination between structures, thus providing structural
information on the orientation of the amino group. Both observed conformers are stabilized by an
O–H · · ·N hydrogen bond in the side chain and a N–H · · · π interaction forming a chain that reinforce
the strength of hydrogen bonds through cooperative effects.
