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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/24431

    Título
    Regeneration of hyaline cartilage promoted by xenogeneic mesenchymal stromal cells embedded within elastin-like recombinamer-based bioactive hydrogels
    Autor
    Pescador, David
    Ibáñez Fonseca, Arturo
    Sánchez Guijo, Fermín
    G. Briñón, Jesús
    Arias Vallejo, Francisco JavierAutoridad UVA Orcid
    Muntión, Sandra
    Hernández, Cristina
    Girotti ., AlessandraAutoridad UVA Orcid
    Alonso Rodrigo, MatildeAutoridad UVA Orcid
    Cañizo, María Consuelo del
    Rodríguez Cabello, José CarlosAutoridad UVA Orcid
    Blanco, Juan Francisco
    Año del Documento
    2017
    Editorial
    Springer
    Descripción
    Producción Científica
    Documento Fuente
    Journal of Materials Science: Materials in Medicine, 2017, 28:115
    Zusammenfassung
    Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELR-based hydrogel alone or with embedded hMSCs. Regeneration outcomes were evaluated after three months by gross assessment, magnetic resonance imaging and computed tomography, showing complete filling of the defect and the de novo formation of hyaline-like cartilage and subchondral bone in the hMSC-treated knees. Furthermore, histological sectioning and staining of every sample confirmed regeneration of the full cartilage thickness and early subchondral bone repair, which was more similar to the native cartilage in the case of the cell-loaded ELR-based hydrogel. Overall histological differences between the two groups were assessed semi-quantitatively using the Wakitani scale and found to be statistically significant (p < 0.05). Immunofluorescence against a human mitochondrial antibody three months post-implantation showed that the hMSCs were integrated into the de novo formed tissue, thus suggesting their ability to overcome the interspecies barrier. Hence, we conclude that the use of xenogeneic MSCs embedded in an ELR-based hydrogel leads to the successful regeneration of hyaline cartilage in osteochondral lesions.
    Palabras Clave
    Cartílago
    Revisión por pares
    SI
    DOI
    10.1007/s10856-017-5928-1
    Patrocinador
    Ministerio de Economía, Industria y Competitividad (Project (MAT2016-78903-R, MAT2016-9 79435-R, MAT2013-42473-R, MAT2013-41723-R and MAT2012-38043)
    Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13, VA313U14 and GRS/516/A/10)
    Patrocinador
    info:eu-repo/grantAgreement/EC/H2020/642687
    info:eu-repo/grantAgreement/EC/H2020/646075
    info:eu-repo/grantAgreement/EC/FP7/278557
    info:eu-repo/grantAgreement/EC/FP7/317306
    Version del Editor
    https://rd.springer.com/article/10.1007/s10856-017-5928-1
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/24431
    Derechos
    openAccess
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    • BIOFORGE - Artículos de revista [89]
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    Regeneration hyaline-Supplementary-Material.pdf
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    Universidad de Valladolid

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