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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/24724

    Título
    Ionic currents in dispersed chemoreceptor cells of the mammalian carotid body
    Autor
    Ureña, J.
    López López, José RamónAutoridad UVA Orcid
    González Martínez, Constancio
    López Barneo, José
    Año del Documento
    1989
    Editorial
    The Rockefeller University Press
    Descripción
    Producción Científica
    Documento Fuente
    Journal of General Physiology, 1989, vol. 93. p. 979-999
    Resumen
    Ionic currents of enzymatically dispersed type 1 and type 11 cells of the carotid body have been studied using the whole cell variant of the patch-clamp technique. Type 11 cells only have a tiny, slowly activating outward potassium cur­ rent. By contrast, in every type 1 chemoreceptor cell studied we found (a) sodium, (b) calcium, and (e) potassium currents. (a) The sodium current has a fast activation time course and an activation threshold at --40 mV. At ali voltages inactivation follows a single exponential time course. The time constant of inactivation is 0.67 ms at O mV. Half steady state inactivation occurs at a membrane potential of --50 mV. (b) The calcium current is almost totally abolished when most of the extemal calcium is replaced by magnesium. The activation threshold of this cur­ rent is at --40 mV and at O mV it reaches a peak amplitude in 6-8 ms. The calcium current inactivates very slowly and only decreases to 27% of the maximal value at the end of 300-ms pulses to 40 mV. The calcium current was about two times larger when barium ions were used as charge carriers instead of calcium ions. Barium ions also shifted 15-20 mV toward negative voltages the conductance vs. voltage curve. Deactivation kinetics of the calcium current follows a biphasic time course well fitted by the sum of two exponentials. At -80 mV the slow com­ ponent has a time constant of 1.3 ± 0.4 ms whereas the fast component, with an amplitude about 20 times larger than the slow component, has a time constant of 0.16 ± 0.03 ms. These results suggest that type 1 cells have predominantly fast deactivating calcium channels. The slow component of the tails may represent the activity of a small population of slowly deactivating calcium channels, although other possibilities are considered. (e) Potassium current seems to be mainly due to the activity of voltage-dependent potassium channels, but a small percentage of calcium-activated channels may also exist. This current activates slowly, reaches a peak amplitude in 5-1O ms, and thereafter slowly inactivates. Inactivation is almost complete in 250-300 ms. The potassium current is reversibly blocked by tetraeth­ ylammonium. Under current-clamp conditions type I cells can spontaneously fire large action potentials.
    Palabras Clave
    Cuerpo carotídeo
    Quimiorreceptores
    Carotid body
    Chemoreceptors
    ISSN
    0022-1295
    Revisión por pares
    SI
    DOI
    10.1085/jgp.93.5.979
    Version del Editor
    https://rupress.org/jgp/article/93/5/979/27758/Ionic-currents-in-dispersed-chemoreceptor-cells-of
    Propietario de los Derechos
    © 1989 The Rockefeller University Press
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/24724
    Derechos
    openAccess
    Aparece en las colecciones
    • IBGM - Artículos de revista [78]
    • VASCUMIT - Artículos de revista [47]
    • DEP06 - Artículos de revista [352]
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    Nombre:
    1989. Ureña et al. J Gen Physiol. 93(5).979-99.pdf
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