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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/29191

    Título
    Tungstate-Targeting of BKαβ1 Channels Tunes ERK Phosphorylation and Cell Proliferation in Human Vascular Smooth Muscle
    Autor
    López López, José RamónAutoridad UVA Orcid
    Fernández Mariño, Ana Isabel
    Cidad Velasco, María Del PilarAutoridad UVA Orcid
    Zafra, Delia
    Nocito, Laura
    Domínguez, Jorge
    Oliván Viguera, Aida
    Köhler, Ralf
    Pérez García, María TeresaAutoridad UVA Orcid
    Valverde, Miguel Ángel
    Guinovart, Joan J.
    Fernández Fernández, José Manuel
    Año del Documento
    2015
    Editorial
    Plos
    Descripción
    Producción Científica
    Documento Fuente
    Plos One, 2015, p. 1-21
    Zusammenfassung
    Despite the substantial knowledge on the antidiabetic, antiobesity and antihypertensive actions of tungstate, information on its primary target/s is scarce. Tungstate activates both the ERK1/2 pathway and the vascular voltage- and Ca2+-dependent large-conductance BKαβ1 potassium channel, which modulates vascular smooth muscle cell (VSMC) proliferation and function, respectively. Here, we have assessed the possible involvement of BKαβ1 channels in the tungstate-induced ERK phosphorylation and its relevance for VSMC proliferation. Western blot analysis in HEK cell lines showed that expression of vascular BKαβ1 channels potentiates the tungstate-induced ERK1/2 phosphorylation in a Gi/o protein-dependent manner. Tungstate activated BKαβ1 channels upstream of G proteins as channel activation was not altered by the inhibition of G proteins with GDPβS or pertussis toxin. Moreover, analysis of Gi/o protein activation measuring the FRET among heterologously expressed Gi protein subunits suggested that tungstate-targeting of BKαβ1 channels promotes G protein activation. Single channel recordings on VSMCs from wild-type and β1-knockout mice indicated that the presence of the regulatory β1 subunit was essential for the tungstate-mediated activation of BK channels in VSMCs. Moreover, the specific BK channel blocker iberiotoxin lowered tungstate-induced ERK phosphorylation by 55% and partially reverted (by 51%) the tungstate-produced reduction of platelet-derived growth factor (PDGF)-induced proliferation in human VSMCs. Our observations indicate that tungstate-targeting of BKαβ1 channels promotes activation of PTX-sensitive Gi proteins to enhance the tungstate-induced phosphorylation of ERK, and inhibits PDGF-stimulated cell proliferation in human vascular smooth muscle.
    Palabras Clave
    Calcio
    Metabolismo
    ISSN
    1932-6203
    Revisión por pares
    SI
    DOI
    10.1371/journal.pone.0118148
    Patrocinador
    Ministerio de Economía, Industria y Competitividad (SAF2012-31089 to JMFF, SAF2012-38140 to MAV, BFU2013-45867-R to JRLL)
    Ministerio de Ciencia e Innovación (BFU 2008-00769 to JG, BFU2010-15898 to MTPG)
    Instituto de Salud Carlos III (RIC RD12/0042/0006, RD12/0042/0014, Red HERACLES)
    Junta de Castilla y León (VA094A11-2 to JRLL)
    Patrocinador
    info:eu-repo/grantAgreement/EC/FP7/CIG-321721
    Version del Editor
    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0118148
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/29191
    Derechos
    openAccess
    Aparece en las colecciones
    • IBGM - Artículos de revista [78]
    • VASCUMIT - Artículos de revista [47]
    • DEP06 - Artículos de revista [352]
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