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dc.contributor.authorRodríguez Méndez, María Luz 
dc.contributor.authorApetrei, Constantin
dc.contributor.authorSaja Sáez, José Antonio de
dc.contributor.authorZurro, Javier
dc.date.accessioned2018-07-16T08:34:18Z
dc.date.issued2012
dc.identifier.citationCatalysts vol. 2 p. 517-531es
dc.identifier.issn2073-4344es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/30702
dc.description.abstractTyrosinase-based biosensors containing a phthalocyanine as electron mediator have been prepared by two different methods. In the first approach, the enzyme and the electron mediator have been immobilized in carbon paste electrodes. In the second method, they have been introduced in an arachidic acid Langmuir-Blodgett nanostructured film that provides a biomimetic environment. The sensing properties of non-nanostructured and nanostructured biosensors towards catechol, catechin and phenol have been analyzed and compared. The enzyme retains the biocatalytic properties in both matrixes. However, the nanostructured biomimetic films show higher values of maximum reaction rates and lowest apparent Michaelis-Menten constants. In both types of sensors, the sensitivity follows the decreasing order catechol > catechin > phenol. The detection limits observed are in the range of 1.8–5.4 μM for Langmuir-Blodgett biosensors and 8.19–8.57 μM for carbon paste biosensors. In summary, it has been demonstrated that the Langmuir-Blodgett films provide a biomimetic environment and nanostructured biosensors show better performances in terms of kinetic, detection limit and stability.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleAdvantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Methodes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.3390/catal2040517es
dc.peerreviewedSIes
dc.description.embargo2022-07-7es
dc.description.lift2022-07-07
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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