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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/30777

    Título
    Optimized architecture for Tyrosinase-containing Langmuir-Blodgett films to detect pyrogallol
    Autor
    Rodríguez Méndez, María LuzAutoridad UVA Orcid
    Pavinato, Felippe J.
    Ramos Fernandes, Edson Giuliani
    Alessio, Priscila
    Constantino, Carlos José Leopoldo
    Saja Saez, José Antonio DeAutoridad UVA Orcid
    Zucolotto, V.
    Oliveira, Osvaldo N.
    Apetrei, Constantin
    Año del Documento
    2011
    Descripción
    Producción Científica
    Documento Fuente
    J. Materials Chemistry vol. 21 p. 4995-5003
    Resumen
    The control of molecular architectures has been a key factor for the use of Langmuir–Blodgett (LB) films in biosensors, especially because biomolecules can be immobilized with preserved activity. In this paper we investigated the incorporation of tyrosinase (Tyr) in mixed Langmuir films of arachidic acid (AA) and a lutetium bisphthalocyanine (LuPc2), which is confirmed by a large expansion in the surface pressure isotherm. These mixed films of AA–LuPc2 + Tyr could be transferred onto ITO and Pt electrodes as indicated by FTIR and electrochemical measurements, and there was no need for crosslinking of the enzyme molecules to preserve their activity. Significantly, the activity of the immobilised Tyr was considerably higher than in previous work in the literature, which allowed Tyr-containing LB films to be used as highly sensitive voltammetric sensors to detect pyrogallol. Linear responses have been found up to 400 μM, with a detection limit of 4.87 × 10−2 μM (n = 4) and a sensitivity of 1.54 μA μM−1 cm−2. In addition, the Hill coefficient (h = 1.27) indicates cooperation with LuPc2 that also acts as a catalyst. The enhanced performance of the LB-based biosensor resulted therefore from a preserved activity of Tyr combined with the catalytic activity of LuPc2, in a strategy that can be extended to other enzymes and analytes upon varying the LB film architecture.
    ISSN
    1463-9076
    Revisión por pares
    SI
    DOI
    10.1039/C0JM03864D
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/30777
    Derechos
    openAccess
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