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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/35330

    Título
    Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers
    Autor
    Flora, Tatjana
    González de Torre, IsraelAutoridad UVA Orcid
    Quintanilla Sierra, LuisAutoridad UVA
    Alonso Rodrigo, MatildeAutoridad UVA Orcid
    Rodríguez Cabello, José CarlosAutoridad UVA Orcid
    Año del Documento
    2018
    Editorial
    Elsevier
    Descripción
    Producción Científica
    Documento Fuente
    European Polymer Journal, 2018, vol. 106. p. 19-29
    Abstract
    A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out. After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof. Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties.
    Palabras Clave
    Control espacial
    Spatial control
    Adhesión celular
    Cell adhesion
    Biofuncionalización superficial
    Surface bio-functionalization
    Ablación láser
    Laser ablation
    Recombinantes tipo elastina
    Elastin-like recombinamers
    ISSN
    0014-3057
    Revisión por pares
    SI
    DOI
    10.1016/j.eurpolymj.2018.06.026
    Patrocinador
    Comisión Europea (NMP-2014-646075, MSCA-ITN-2014-642687)
    Ministerio de Economía, Industria y Competitividad (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R and MAT2016-79435-R)
    Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. Project VA015U16)
    Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León
    Version del Editor
    https://www.sciencedirect.com/science/article/pii/S0014305717322061
    Propietario de los Derechos
    © 2019 Elsevier
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/35330
    Derechos
    openAccess
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    • BIOFORGE - Artículos de revista [89]
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