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dc.contributor.authorLorenzo Martín, Elvira
dc.contributor.authorGallego Muñoz, Patricia 
dc.contributor.authorMar Sardaña, Santiago
dc.contributor.authorFernández Martínez, Itziar 
dc.contributor.authorCidad Velasco, María del Pilar
dc.contributor.authorMartínez García, María del Carmen 
dc.date.accessioned2019-07-26T09:27:29Z
dc.date.available2019-07-26T09:27:29Z
dc.date.issued2019
dc.identifier.citationExperimental Eye Research, 2019, vol. 186es
dc.identifier.issn0014-4835es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/37291
dc.descriptionProducción Científicaes
dc.description.abstractThe extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans. We monitored the corneal wound healing process after alkali burns in rabbits. We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency. After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s. The eyes were evaluated under a surgical microscope at 1, 3, and 6 months after burning. At each time point, the clinical conditions of the burned and control corneas were observed. The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy. Corneal light transmittance was also measured. Myofibroblasts presence was analyzed by immunohistochemistry. mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction. One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area. Corneal opacities were present with attendant loss of light transmittance. Collagen and proteoglycan mRNA expression levels were up-regulated. After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels. After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture. This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning. Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationExtracellular matrixes
dc.subject.classificationMatriz extracelulares
dc.subject.classificationCollagen type IIIes
dc.subject.classificationColágeno tipo IIIes
dc.subject.classificationDecorines
dc.subject.classificationDecorinaes
dc.subject.classificationCorneal wound healinges
dc.subject.classificationCicatrización de herida corneales
dc.titleDynamic changes of the extracellular matrix during corneal wound healinges
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2019 Elsevieres
dc.identifier.doihttps://doi.org/10.1016/j.exer.2019.107704es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0014483519302040es
dc.peerreviewedSIes
dc.description.projectInstituto de Salud Carlos III (Health Research Fund PI15/01906 )es
dc.description.projectMinisterio de Economía, Industria y Competitividad ( project BFU2016-75360-R)es
dc.description.projectJunta de Castilla y León (Project VA114P17)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/acceptedVersiones


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