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    Título
    Genome scale metabolic modeling reveals the metabolic potential of three Type II methanotrophs of the genus Methylocystis
    Autor
    Bordel Velasco, SergioAutoridad UVA Orcid
    Rodríguez Muñoz, YadiraAutoridad UVA Orcid
    Hakobyan, Anna
    Rodríguez Rodríguez, Elisa
    Lebrero Fernández, RaquelAutoridad UVA Orcid
    Muñoz Torre, RaúlAutoridad UVA Orcid
    Año del Documento
    2019
    Editorial
    Elsevier
    Descripción
    Producción Científica
    Documento Fuente
    Metabolic Engineering Volume 54, 2019, Pages 191-199
    Résumé
    Genome Scale Metabolic Models (GSMMs) of the recently sequenced Methylocystis hirsuta and two other methanotrophs from the genus Methylocystis have been reconstructed. These organisms are Type II methanotrophs with the ability of accumulating Polyhydroxyalkanoates under nutrient limiting conditions. For the first time, GSMMs have been reconstructed for Type II methanotrophs. These models, combined with experimental biomass and PHB yields of Methylocystis hirsuta, allowed elucidating the methane oxidation mechanism by the enzyme pMMO (particulate methane monooxygenase) in these organisms. In contrast to Type I methanotrophs, which use the “direct coupling mechanism”, Type II methanotrophs appear to use the so called “redox arm mechanism”. The utilization of the “redox arm mechanism”, which involves the coupling between methane oxidation and complex I of the respiratory chain, was confirmed by inhibition of complex I with catechol. Utilization of the “redox arm” mechanism leads to lower biomass yields on methane compared to Type I methanotrophs. However, the ability of Type II methanotrophs to redirect high metabolic carbon fluxes towards acetoacetyl-CoA under nitrogen limiting conditions makes these organisms promising platforms for metabolic engineering.
    Materias (normalizadas)
    Genome
    Palabras Clave
    Genoma
    ISSN
    1096-7176
    Revisión por pares
    SI
    DOI
    10.1016/j.ymben.2019.04.001
    Patrocinador
    Marie Curie grant H2020-MSCA-IF-2016 CH4BioVal (GA nº 750126).
    Junta de Castilla y León (Ref. Project VA281P18)
    Ministerio de Ciencia e Innovación (Proyect CLU 2017-09, CTM2015-70442-R)
    Patrocinador
    info:eu-repo/grantAgreement/EC/H2020/750126
    Version del Editor
    https://www.sciencedirect.com/science/article/pii/S1096717618304592
    Propietario de los Derechos
    © 2019 Elsevier
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/38025
    Tipo de versión
    info:eu-repo/semantics/SubmittedVersion
    Derechos
    openAccess
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    • DEP48 - Artículos de revista [265]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalExcepté là où spécifié autrement, la license de ce document est décrite en tant que Attribution-NonCommercial-NoDerivatives 4.0 Internacional

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