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dc.contributor.authorMata Sampedro, Ana de la
dc.contributor.authorMateos Timoneda, Miguel Ángel
dc.contributor.authorNieto Miguel, Teresa
dc.contributor.authorGalindo de la Rosa, Sara 
dc.contributor.authorLópez Paniagua, Marina 
dc.contributor.authorPlanell, Josep A.
dc.contributor.authorEngel, Elisabeth
dc.contributor.authorCalonge Cano, Margarita 
dc.date.accessioned2020-01-24T11:01:57Z
dc.date.available2020-01-24T11:01:57Z
dc.date.issued2019
dc.identifier.citationColloids and Surfaces B: Biointerfaces: 2019. 177: 121-129es
dc.identifier.issn0927-7765es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/40348
dc.description.abstractLimbal epithelial stem cells (LESCs) are responsible for the renewal of corneal epithelium. Cultivated limbal epithelial transplantation is the current treatment of choice for restoring the loss or dysfunction of LESCs. To perform this procedure, a substratum is necessary for in vitro culturing of limbal epithelial cells and their subsequent transplantation onto the ocular surface. In this work, we evaluated poly-L/DL-lactic acid 70:30 (PLA) films functionalized with type IV collagen (col IV) as potential in vitro carrier substrata for LESCs. We first demonstrated that PLA-col IV films were biocompatible and suitable for the proliferation of human corneal epithelial cells. Subsequently, limbal epithelial cell suspensions, isolated from human limbal rings, were cultivated using culture medium that did not contain animal components. The cells adhered significantly faster to PLA-col IV films than to tissue culture plastic (TCP). The mRNA expression levels for the LESC specific markers, K15, P63α and ABCG2 were similar or greater (significantly in the case of K15) in limbal epithelial cells cultured on PLA-col IV films than limbal epithelial cells cultured on TCP. The percentage of cells expressing the corneal (K3, K12) and the LESC (P63α, ABCG2) specific markers was similar for both substrata. These results suggest that the PLA-col IV films promoted LESC attachment and helped to maintain their undifferentiated stem cell phenotype. Consequently, these substrata offer an alternative for the transplantation of limbal cells onto the ocular surface.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.subject.classificationCorneal epithelium, collagen IV, limbal stem cells, polylactic acid, tissue engineeringes
dc.titlePoly-l/dl-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cellses
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holderELSEVIER, Radarweg 29, 1043 NX Amsterdam, The Netherlandses
dc.identifier.doi10.1016/j.colsurfb.2019.01.054es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776519300542?via%3Dihubes
dc.relation.publisherversionhttps://doi.org/10.1016/j.colsurfb.2019.01.054es
dc.identifier.publicationfirstpage121es
dc.identifier.publicationlastpage129es
dc.identifier.publicationtitleColloids and Surfaces B: Biointerfaceses
dc.identifier.publicationvolume177es
dc.peerreviewedSIes
dc.description.projectThis work was supported by the Carlos III National Institute of Health, Spain (CIBER-BBN and Spanish Network on Cell Therapy, (TerCel RD12/0019/0036), MINECO/FEDER, EU), and the Castilla y León Regional Government, Spain (Regional Center for Regenerative Medicine and Cell Therapy, SAN673/VA/28/08 and SAN126/VA11/09).es
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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