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    • Edición Génica para el Estudio de Canales Iónicos Vasculares y Proteínas Mitocondriales (VASCUMIT)
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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/44599

    Título
    Characterization of ion channels involved in the proliferative response of femoral artery smooth muscle cells
    Autor
    Cidad Velasco, María Del PilarAutoridad UVA Orcid
    Moreno Domínguez, Alejandro
    Novensá, Laura
    Roqué, Mercé
    Barquín, Leire
    Heras i Fortuny, Maria Magdalena
    Pérez García, María TeresaAutoridad UVA Orcid
    López López, José RamónAutoridad UVA Orcid
    Año del Documento
    2010
    Editorial
    American Heart Association
    Descripción
    Producción Científica
    Documento Fuente
    Arteriosclerosis, Thrombosis, and Vascular Biology, 2010, vol. 30, n. 6. p. 1203-1211
    Abstract
    Objective: Vascular smooth muscle cells (VSMCs) contribute significantly to occlusive vascular diseases by virtue of their ability to switch to a noncontractile, migratory, and proliferating phenotype. Although the participation of ion channels in this phenotypic modulation (PM) has been described previously, changes in their expression are poorly defined because of their large molecular diversity. We obtained a global portrait of ion channel expression in contractile versus proliferating mouse femoral artery VSMCs, and explored the functional contribution to the PM of the most relevant changes that we observed. Methods and Results: High-throughput real-time polymerase chain reaction of 87 ion channel genes was performed in 2 experimental paradigms: an in vivo model of endoluminal lesion and an in vitro model of cultured VSMCs obtained from explants. mRNA expression changes showed a good correlation between the 2 proliferative models, with only 2 genes, Kv1.3 and Kvβ2, increasing their expression on proliferation. The functional characterization demonstrates that Kv1.3 currents increased in proliferating VSMC and that their selective blockade inhibits migration and proliferation. Conclusion: These findings establish the involvement of Kv1.3 channels in the PM of VSMCs, providing a new therapeutical target for the treatment of intimal hyperplasia.
    Palabras Clave
    Gene expression
    Expresión génica
    Ion channels
    Canales iónicos
    Restenosis
    Vascular smooth muscle
    Músculo liso vascular
    ISSN
    1524-4636
    Revisión por pares
    SI
    DOI
    10.1161/ATVBAHA.110.205187
    Patrocinador
    Ministerio de Sanidad, Consumo y Bienestar Social - Instituto de Salud Carlos III (grants R006/009, FS041139-0 and PI041044)
    Ministerio de Ciencia, Innovación y Universidades (grants BFU2004-05551 and BFU2007-61524)
    Junta de Castilla y León (grant GR242)
    Version del Editor
    https://www.ahajournals.org/doi/10.1161/ATVBAHA.110.205187
    Propietario de los Derechos
    © 2010 American Heart Association
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/44599
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Collections
    • DEP06 - Artículos de revista [352]
    • IBGM - Artículos de revista [78]
    • VASCUMIT - Artículos de revista [47]
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    Universidad de Valladolid

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