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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45031

    Título
    Transcriptomic analysis of calcium remodeling in colorectal cancer
    Autor
    Pérez Riesgo, EnriqueAutoridad UVA
    González Gutiérrez, LucíaAutoridad UVA
    Ubierna, Daniel
    Acedo, Alberto
    Moyer, Mary P.
    Núñez Llorente, LucíaAutoridad UVA
    Villalobos Jorge, Carlos
    Año del Documento
    2017
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    International Journal of Molecular Sciences, 2017, vol. 18, n. 5. 23 p.
    Abstract
    Colorectal cancer (CRC) cells undergo the remodeling of intracellular Ca2+ homeostasis, which contributes to cancer hallmarks such as enhanced proliferation, invasion and survival. Ca2+ remodeling includes critical changes in store-operated Ca2+ entry (SOCE) and Ca2+ store content. Some changes have been investigated at the molecular level. However, since nearly 100 genes are involved in intracellular Ca2+ transport, a comprehensive view of Ca2+ remodeling in CRC is lacking. We have used Next Generation Sequencing (NGS) to investigate differences in expression of 77 selected gene transcripts involved in intracellular Ca2+ transport in CRC. To this end, mRNA from normal human colonic NCM460 cells and human colon cancer HT29 cells was isolated and used as a template for transcriptomic sequencing and expression analysis using Ion Torrent technology. After data transformation and filtering, exploratory analysis revealed that both cell types were well segregated. In addition, differential gene expression using R and bioconductor packages show significant differences in expression of selected voltage-operated Ca2+ channels and store-operated Ca2+ entry players, transient receptor potential (TRP) channels, Ca2+ release channels, Ca2+ pumps, Na+/Ca2+ exchanger isoforms and genes involved in mitochondrial Ca2+ transport. These data provide the first comprehensive transcriptomic analysis of Ca2+ remodeling in CRC.
    Palabras Clave
    Colorectal cancer
    Cáncer colorrectal
    Calcium
    Calcio
    Transcriptomics
    Transcriptoma
    RNA-sequencing
    Secuenciación de ARN
    ISSN
    1422-0067
    Revisión por pares
    SI
    DOI
    10.3390/ijms18050922
    Patrocinador
    Ministerio de Economía, Industria y Competitividad (grant BFU2015-70131-R)
    Junta de Castilla y León (grant VA145U13)
    Version del Editor
    https://www.mdpi.com/1422-0067/18/5/922
    Propietario de los Derechos
    © 2017 MDPI
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/45031
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • CFC - Artículos de Revista [38]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional

    Universidad de Valladolid

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