Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45033
Título
Delphinidin reduces glucose uptake in mice jejunal tissue and human intestinal cells lines through FFA1/GPR40
Autor
Año del Documento
2017
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
International Journal of Molecular Sciences, 2017, vol. 18, n. 4. 18 p.
Resumen
Anthocyanins are pigments with antihyperglycemic properties, and they are potential candidates for developing functional foods for the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism of these beneficial effects of anthocyanins are, however, hard to explain, given their very low bioavailability due to poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect of the anthocyanidin delphinidin over intestinal glucose absorption. We show the direct effects of delphinidin on the intestine using jejunum samples from RF/J mice, and the human intestinal cell lines HT-29, Caco-2, and NCM460. By the use of specific pharmacological antagonists, we determined that delphinidin inhibits glucose absorption in both mouse jejunum and a human enterocytic cell line in a FFA1-dependent manner. Delphinidin also affects the function of sodium-glucose cotransporter 1 (SGLT1). Intracellular signaling after FFA1 activation involved cAMP increase and cytosolic Ca2+ oscillations originated from intracellular Ca2+ stores and were followed by store-operated Ca2+ entry. Taken together, our results suggest a new GPR-40 mediated local mechanism of action for delphinidin over intestinal cells that may in part explain its antidiabetic effect. These findings are promising for the search for new prevention and pharmacological treatment strategies for DM2 management.
Palabras Clave
Delphinidin
Delfinidina
Anthocyanins
Antocianina
Glucose
Glucosa
ISSN
1422-0067
Revisión por pares
SI
Patrocinador
INNOVA CORFO (grant 12IDL2-16254)
Comisión Nacional de Investigación Científica y Tecnológica (grant 21130644)
Ministerio de Economía, Industria y Competitividad (grant BFU2015-70131-R)
Comisión Nacional de Investigación Científica y Tecnológica (grant 21130644)
Ministerio de Economía, Industria y Competitividad (grant BFU2015-70131-R)
Version del Editor
Propietario de los Derechos
© 2017 MDPI
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Ficheros en el ítem
La licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional