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Título
Calcium dynamics and resting transcriptional activity regulates prolactin gene expression
Autor
Año del Documento
2002
Editorial
Oxford University Press
Descripción
Producción Científica
Documento Fuente
Endocrinology, 2002, vol. 143, n. 9. p. 3548–3554
Abstract
Research on the regulation of hormone gene expression by calcium signaling is hampered by the difficulty of monitoring both parameters within the same individual, living cells. Here we achieved concurrent, dynamic measurements of both intracellular Ca2+ concentration ([Ca2+]i) and prolactin (PRL) gene promoter activity in single, living pituitary cells. Cells were transfected with the luciferase reporter gene under control of the PRL promoter and subjected to bioluminescence and fluorescence imaging before and after presentation of TSH-releasing hormone (TRH), a prototypic regulator of PRL secretion and gene expression that induces a transient Ca2+ release, followed by sustained Ca2+ influx. We found that cells displaying specific photonic emissions (i.e. mammotropes) showed heterogeneous calcium and transcriptional responses to TRH. Transcriptionally responsive cells always exhibited a TRH-induced [Ca2+]i increase. In addition, transcriptional responses were related to the rate of Ca2+ entry but not Ca2+ release. Finally, cells lacking transcriptional responses (but showing [Ca2+]i rises) exhibited larger levels of resting PRL promoter activity than transcriptionally responsive cells. Thus, our results suggest that the sustained entry of Ca2+ induced by TRH (but not the Ca2+ release) regulates transcriptional responsiveness. Superimposed on this regulation, the previous, resting PRL promoter activity also controls transcriptional responses.
Palabras Clave
Gene expression
Expresión génica
Prolactin
Prolactina
ISSN
1945-7170
Revisión por pares
SI
Patrocinador
National Institutes of Health (grant DK-38215)
Fondo de Investigaciones Sanitarias (grant FIS 01/0769)
Fondo de Investigaciones Sanitarias (grant FIS 01/0769)
Version del Editor
Propietario de los Derechos
© 2002 Oxford University Press
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Collections
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