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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/45074

    Título
    Calcium dynamics and resting transcriptional activity regulates prolactin gene expression
    Autor
    Villalobos Jorge, Carlos
    Núñez Llorente, LucíaAutoridad UVA
    Faught, William J.
    Leaumont, David C.
    Boockfor, Fredric R.
    Frawley, L. Stephen
    Año del Documento
    2002
    Editorial
    Oxford University Press
    Descripción
    Producción Científica
    Documento Fuente
    Endocrinology, 2002, vol. 143, n. 9. p. 3548–3554
    Abstract
    Research on the regulation of hormone gene expression by calcium signaling is hampered by the difficulty of monitoring both parameters within the same individual, living cells. Here we achieved concurrent, dynamic measurements of both intracellular Ca2+ concentration ([Ca2+]i) and prolactin (PRL) gene promoter activity in single, living pituitary cells. Cells were transfected with the luciferase reporter gene under control of the PRL promoter and subjected to bioluminescence and fluorescence imaging before and after presentation of TSH-releasing hormone (TRH), a prototypic regulator of PRL secretion and gene expression that induces a transient Ca2+ release, followed by sustained Ca2+ influx. We found that cells displaying specific photonic emissions (i.e. mammotropes) showed heterogeneous calcium and transcriptional responses to TRH. Transcriptionally responsive cells always exhibited a TRH-induced [Ca2+]i increase. In addition, transcriptional responses were related to the rate of Ca2+ entry but not Ca2+ release. Finally, cells lacking transcriptional responses (but showing [Ca2+]i rises) exhibited larger levels of resting PRL promoter activity than transcriptionally responsive cells. Thus, our results suggest that the sustained entry of Ca2+ induced by TRH (but not the Ca2+ release) regulates transcriptional responsiveness. Superimposed on this regulation, the previous, resting PRL promoter activity also controls transcriptional responses.
    Palabras Clave
    Gene expression
    Expresión génica
    Prolactin
    Prolactina
    ISSN
    1945-7170
    Revisión por pares
    SI
    DOI
    10.1210/en.2002-220266
    Patrocinador
    National Institutes of Health (grant DK-38215)
    Fondo de Investigaciones Sanitarias (grant FIS 01/0769)
    Version del Editor
    https://academic.oup.com/endo/article/143/9/3548/2880874
    Propietario de los Derechos
    © 2002 Oxford University Press
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/45074
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • CFC - Artículos de Revista [38]
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    Attribution-NonCommercial-NoDerivs 3.0 UnportedLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivs 3.0 Unported

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