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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/46637

    Título
    Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates
    Autor
    Paniagua García, Ana I.
    Hijosa Valsero, María
    Garita Cambronero, Jerson
    Coca Sanz, MónicaAutoridad UVA Orcid
    Díez Antolínez, Rebeca
    Año del Documento
    2019
    Editorial
    Elsevier
    Descripción
    Producción Científica
    Documento Fuente
    Microchemical Journal, 2019, vol. 150, p. 104147
    Resumen
    Lignocellulosic agro-food wastes are regarded as interesting carbohydrate sources for acetone-butanol-ethanol (ABE) fermentation. However, the physicochemical and enzymatic pretreatments applied to release their sugars generate inhibitory compounds that hinder the fermentation. The release of inhibitory compounds in the hydrolysates of four agro-food industrial wastes [apple pomace (AP), potato peel (PP), brewers' spent grain (BSG) and coffee silverskin (CS)] obtained after various chemical pretreatments (acid, alkali, organic solvents and surfactant pretreatments) was analyzed. Sixty-seven potential inhibitors were identified by gas chromatography–mass spectrometry (GC–MS) and were classified into non-aromatic compounds (aliphatic acids, nitrogen-containing compounds, furans and fatty acids) and aromatic compounds (phenolics and non-phenolics). Then, a high performance liquid chromatography method with diode array detection (HPLC-DAD) was developed and validated for the quantification of the main potential inhibitors identified in the hydrolysates (i.e. gallic, 3,4-dihydroxybenzoic, 2,5-dihydroxibenzoic, 4-hydroxybenzoic, 3-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, and ferulic acids, vanillin, syringaldehyde and caffeine). The proposed HPLC-DAD method was simple, fast and robust and allowed the direct injection of samples without previous treatment, enabling the simultaneous quantification of the abovementioned compounds for the first time. The method was successfully applied to the analysis of AP, PP, BSG and CS hydrolysates.
    Materias Unesco
    23 Química
    Palabras Clave
    Residuos industriales agroalimentarios
    Compuesto fenólico
    Hidrolizado
    ISSN
    0026-265X
    Revisión por pares
    SI
    DOI
    10.1016/j.microc.2019.104147
    Patrocinador
    Instituto Nacional de Investigaciones Agronómicas (INIA) (DOC-INIA, grant number DOC 2013-010)
    H2020-LCE-2015 Waste2Fuels project (Sustainable production of next generation biofuels from waste streams - Waste2Fuels. GA - 654623)
    Patrocinador
    info:eu-repo/grantAgreement/EC/H2020/654623
    Version del Editor
    https://www.sciencedirect.com/science/article/pii/S0026265X1930877X
    Propietario de los Derechos
    © 2019 Elsevier
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/46637
    Tipo de versión
    info:eu-repo/semantics/acceptedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • IPS - Artículos de revista [157]
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    Development-and-validation-HPLC-DAD.pdf
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    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional

    Universidad de Valladolid

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