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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/47008

    Título
    Acidic triterpenes compromise growth and survival of astrocytoma cell lines by regulating reactive oxygen species accumulation
    Autor
    Martín Martín, Rubén
    Carvalho Tavares, Juliana
    Ibeas, Elvira
    Hernández Garrido, MaritaAutoridad UVA Orcid
    Ruiz Gutierrez, Valentina
    Nieto Callejo, María Luisa
    Año del Documento
    2007
    Editorial
    American Association for Cancer Research
    Descripción
    Producción Científica
    Documento Fuente
    Cancer Research, 2007, vol. 67, n. 8. p. 3741-3751
    Resumen
    Several studies have shown how pentacyclic triterpenes can inhibit proliferation and induce apoptosis of some tumor cell lines; however, its effect on astrocytic tumors, one of the most malignant forms of cancer, has rarely been reported. The aim of this study was to examine how the pentacyclic triterpenes, oleanolic acid and maslinic acid, isolated from olive juice, affected astrocytoma cell morphology and survival. Cell proliferation was inhibited in 1321N1 astrocytoma cells by using 1 to 50 μmol/L of either oleanolic acid or maslinic acid, with an average IC50 of 25 μmol/L. Growth inhibition led to morphologic and cytoskeletal alterations associated with the loss of stellate morphology and characterized by a retraction of the cytoplasm and collapse of actin stress fibers. Using 4′,6-diamidino-2-phenylindole and Annexin V, we showed that astrocytoma cell death induced by oleanolic acid or maslinic acid were mainly due to apoptotic events. Furthermore, we showed that caspase-3 is activated as a consequence of triterpene treatment. Finally, we found that exposure of the cells to oleanolic acid or maslinic acid resulted in a significant increase of intracellular reactive oxygen species, followed by loss of mitochondrial membrane integrity. Importantly, enzymatic scavengers, such as catalase, or phenolic antioxidants, such as butylated hydroxytoluene, rescued cells from the triterpene-mediated apoptosis, suggesting that the potential therapeutic effect of these acidic triterpenes is dependent on oxidative stress. Our data show that acidic triterpenes play a major role in 1321N1 astrocytoma morphology and viability and support the conclusion that oleanolic acid and maslinic acid may thus be promising new agents in the management of astrocytomas.
    Materias Unesco
    3201.01 Oncología
    Palabras Clave
    Triterpenes
    Triterpenos
    Astrocytoma cells
    Astrocitomas
    ISSN
    1538-7445
    Revisión por pares
    SI
    DOI
    10.1158/0008-5472.CAN-06-4759
    Patrocinador
    Plan Nacional de Salud y Farmacia (grant SAF2005-01242)
    Comisión Interministerial de Ciencia y Tecnología (grant AGL-2005-0572)
    Junta de Andalucía (grant FS/FN/ARM:AGR163)
    Version del Editor
    https://cancerres.aacrjournals.org/content/67/8/3741
    Propietario de los Derechos
    © 2007 American Association for Cancer Research
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/47008
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP06 - Artículos de revista [352]
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