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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/49112

    Título
    Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces
    Autor
    Salvo Comino, CoralAutoridad UVA
    García Hernández, CeliaAutoridad UVA
    García Cabezón, Ana CristinaAutoridad UVA Orcid
    Rodríguez Méndez, María LuzAutoridad UVA Orcid
    Año del Documento
    2020
    Editorial
    Elsevier
    Descripción
    Producción Científica
    Documento Fuente
    Bioelectrochemistry, 2020, vol. 132, 107407
    Resumen
    The performance of electrochemical laccase-based biosensors can be improved by immobilizing the enzyme on composite Layer-by-Layer (LbL) supports in which materials with complementary functions are combined. LbL films are formed by layers combining an electrocatalytic material which favors electron transfer (sulfonated copper phthalocyanine, CuPcS(−)), an ionic liquid which enhances the electrical conductivity of the layers (1-butyl-3-methylimidazolium tetrafluoroborate, IL(+)) and a material able to promote enzyme immobilization (chitosan, CHI(+)). Composite films with different structures have been demonstrated to be efficient electrocatalysts, producing an increase in the magnitude of the responses towards catechol. The most intense and reproducible electrocatalytic effect was observed when a layer of the CuPcS(−) was placed on top of a layer formed by a mixture of CHI(+) + IL(+) to obtain [CHI(+) + IL(+)|CuPcS(−)]2 films. Biosensors with laccase immobilized on the surface of the LbL layers [CHI(+) + IL(+)|CuPcS(−)]2|Lac showed mediated electron transfer between the redox enzyme and the film and a reproducibility of device-to-device performance of 4.1%. The amperometric biosensor showed a sensitivity of 0.237 A·M−1 and a linear detection range from 2.4 μM to 26 μM for catechol. The excellent Limit of detection (LOD) of 8.96·10−10 M (3·σ /m) is one order of magnitude lower than that obtained in similar studies. A Michaelis-Menten constant of 3.16 μM confirms excellent enzyme-substrate affinity.
    Palabras Clave
    Laccases
    Lacasas
    Biosensors
    Biosensores
    Catechol
    Catecol
    Phthalocyanine
    Ftalocianina
    ISSN
    1567-5394
    Revisión por pares
    SI
    DOI
    10.1016/j.bioelechem.2019.107407
    Patrocinador
    Ministerio de Economía, Industria y Competitividad - Fondo Europeo de Desarrollo Regional (projects AGL2015-67482-R and RTI2018_097990-B-100)
    Junta de Castilla y León - Fondo Europeo de Desarrollo Regional (project VA275P18)
    Version del Editor
    https://www.sciencedirect.com/science/article/pii/S1567539419301495?via%3Dihub
    Propietario de los Derechos
    © 2020 Elsevier
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/49112
    Tipo de versión
    info:eu-repo/semantics/acceptedVersion
    Derechos
    openAccess
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    • BioEcoUVa - Artículos de revista [195]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalLa licencia del ítem se describe como Attribution-NonCommercial-NoDerivatives 4.0 Internacional

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