• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Navegar

    Todo o repositórioComunidadesPor data do documentoAutoresAssuntosTítulos

    Minha conta

    Entrar

    Estatística

    Ver as estatísticas de uso

    Compartir

    Ver item 
    •   Página inicial
    • PRODUÇÃO CIENTÍFICA
    • Departamentos
    • Dpto. Didáctica de las Ciencias Experimentales, Sociales y de la Matemática
    • DEP97 - Artículos de revista
    • Ver item
    •   Página inicial
    • PRODUÇÃO CIENTÍFICA
    • Departamentos
    • Dpto. Didáctica de las Ciencias Experimentales, Sociales y de la Matemática
    • DEP97 - Artículos de revista
    • Ver item
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano

    Exportar

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/53816

    Título
    Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8
    Autor
    Ramírez Arcos, Sandra
    Moreno Albiger, Renata
    Zafra Amorós, Olga
    Castán García, Pablo
    Valles Rapp, CristinaAutoridad UVA Orcid
    Berenguer Carlos, José
    Año del Documento
    2000
    Editorial
    American Society for Microbiology
    Descripción
    Producción Científica
    Documento Fuente
    Journal of Bacteriology, Vol. 182, Nº. 8, 2020, p. 2179-2183
    Resumo
    Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes,narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed.
    Materias (normalizadas)
    Biologia molecular
    Bacterias
    Respiración
    Nitratos
    Palabras Clave
    Nitrate respiration
    Thermophilic
    Thermus
    ISSN
    0021-9193
    Revisión por pares
    SI
    DOI
    10.1128/JB.182.8.2179-2183.2000
    Patrocinador
    Comisión Interministerial de Ciencia y Tecnologı́a (Project BIO98-0183)
    Ministerio de Educación, Cultura y Deporte y Unión Europea (Proyect 2FD97-0127- C02-01)
    Version del Editor
    https://journals.asm.org/doi/10.1128/jb.182.8.2179-2183.2000?permanently=true&
    Propietario de los Derechos
    © American Society for Microbiology
    Idioma
    spa
    URI
    https://uvadoc.uva.es/handle/10324/53816
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP97 - Artículos de revista [131]
    Mostrar registro completo
    Arquivos deste item
    Nombre:
    Two nitrate.pdf
    Tamaño:
    516.5Kb
    Formato:
    Adobe PDF
    Thumbnail
    Visualizar/Abrir
    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalExceto quando indicado o contrário, a licença deste item é descrito como Attribution-NonCommercial-NoDerivatives 4.0 Internacional

    Universidad de Valladolid

    Powered by MIT's. DSpace software, Version 5.10