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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/53850

    Título
    Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
    Autor
    Ares Sacristán, Ana MaríaAutoridad UVA
    Martín Gómez, María TeresaAutoridad UVA
    Toribio Recio, LauraAutoridad UVA Orcid
    Bernal del Nozal, JoséAutoridad UVA Orcid
    Año del Documento
    2022
    Editorial
    Springer
    Descripción
    Producción Científica
    Documento Fuente
    Food Analytical Methods, volume 15, 2022, pages 2172–2180
    Abstract
    Bee pollen is one of the hive products that is of most interest today due to its multiple beneficial health properties, making it an increasingly popular food supplement. Bee pollen contains many bioactive compounds, such as fatty acids, vitamins, minerals, proteins, and amino acids, among others. In the present study, the free amino acid content was determined in bee pollen by using liquid chromatography coupled to a fluorescence detector. Sample treatment consisted of a solvent extraction of the free amino acids with ultrapure water and a further centrifugation of the extract, which was repeated twice. After that, it was necessary to perform a pre-column derivatization of the amino acids using a combination of two reagents (o-phthalaldehyde and 9-fluorenylmethyl chloroformate) prior to their separation in a Gemini® C18 reverse phase column in gradient elution mode. The analytical performance was evaluated, and several commercial bee pollen samples were analyzed. Significant differences in the free amino acid profile and concentration, which ranged between 19 and 192 mg/g, were observed depending on the botanical origin of the samples.
    Materias Unesco
    23 Química
    2301 Química Analítica
    Palabras Clave
    Bee pollen
    Derivatization
    Fluorescence detection
    Free amino acid
    Liquid chromatography
    Solvent extraction
    ISSN
    1936-9751
    Revisión por pares
    SI
    DOI
    10.1007/s12161-022-02281-8
    Patrocinador
    Ministerio de Economía y Competitividad y el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (project number RTA 2015–00013-C03-03)
    Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE
    Version del Editor
    https://link.springer.com/article/10.1007/s12161-022-02281-8
    Propietario de los Derechos
    © 2022 The Authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/53850
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Collections
    • CINQUIMA - Artículos de revista [112]
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    Atribución 4.0 InternacionalExcept where otherwise noted, this item's license is described as Atribución 4.0 Internacional

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