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dc.contributor.authorKatsinas, Nicolaos 
dc.contributor.authorRodríguez Rojo, Soraya
dc.contributor.authorEnriquez De Salamanca Aladro, Amalia 
dc.date.accessioned2022-09-23T11:17:35Z
dc.date.available2022-09-23T11:17:35Z
dc.date.issued2021
dc.identifier.citationAntioxidants, 2021, Vol. 10, Nº. 7, 25 pp.es
dc.identifier.issn2076-3921es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/55613
dc.descriptionProducción Científicaes
dc.description.abstractOxidative- and inflammatory-related ocular surface diseases have high prevalence and are an emerging issue in ophthalmology. Olive pomace (OP) is the olive oil’s industry main by-product, and is potentially environmentally hazardous. Nevertheless, it contains phenolic compounds with important bioactivities, like oleuropein (OL) and hydroxytyrosol (HT). The antioxidant and anti-inflammatory effects of four OP extracts (CONV, OPT(1–3)), pure OL and HT, and mixtures thereof were screened on human corneal (HCE) and conjunctival epithelial (IM-ConjEpi) cells. CONV was conventionally extracted, while OPT(1–3) were produced by pressurized liquid extraction. Thanks to their improved activity, CONV and OPT3 (HT-enriched) were selected for dose-dependent studies. Cells were stimulated with tumor necrosis factor-α or ultraviolet-B radiation, measuring interleukin (IL)-1β, IL-6, IL-8, and IL-17A as well as interferon γ-induced protein [IP]-10 secretion or intracellular ROS production, respectively. On HCE, both extracts and HT inhibited the secretion of most measured ILs, demonstrating a strong anti-inflammatory effect; while in IM-ConjEpi, all samples decreased IP-10 secretion. Moreover, HT, OL, and both extracts showed strong dose-dependent antioxidant activity in both cell lines. Compared with CONV, OPT3 was active at lower concentrations, demonstrating that intensified extraction techniques are selective towards targeted biomarkers. Hence, a high-value application as potential ocular surface therapy was proposed for the OP valorization.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherMDPIes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectCornea - Diseaseses
dc.subjectConjunctiva - Diseaseses
dc.subjectOjo - Enfermedades y defectos - Tratamientoes
dc.subjectOjo secoes
dc.subjectAceite de olivaes
dc.subjectAceite de oliva - Análisises
dc.subjectAntioxidantes
dc.subject.classificationOlive pomacees
dc.subject.classificationPhenolic extractses
dc.titleOlive pomace phenolic compounds and extracts can inhibit inflammatory- and oxidative-related diseases of human ocular surface epitheliumes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2021 The Authorses
dc.identifier.doi10.3390/antiox10071150es
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/10/7/1150es
dc.identifier.publicationfirstpage1es
dc.identifier.publicationissue7es
dc.identifier.publicationlastpage25es
dc.identifier.publicationtitleAntioxidantses
dc.identifier.publicationvolume10es
dc.peerreviewedSIes
dc.description.projectEuropean Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Initial Training Network (ITN) “IT-DED3” (H2020-MSCA-ITN-2017) grant agreement No. 765608es
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/765608
dc.identifier.essn2076-3921es
dc.rightsAtribución 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco3201.09 Oftalmologíaes


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