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    • Dpto. Bioquímica y Biología Molecular y Fisiología
    • DEP06 - Artículos de revista
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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/58458

    Título
    Mitochondrial Ca2+ dynamics in MCU knockout C. elegans worms
    Autor
    Álvarez Illera, María Pilar
    García Casas, PalomaAutoridad UVA
    Fonteriz García, Rosalba InésAutoridad UVA Orcid
    Montero Zoccola, María TeresaAutoridad UVA Orcid
    Álvarez Martín, JavierAutoridad UVA Orcid
    Año del Documento
    2020
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    International Journal of Molecular Sciences, 2020, Vol. 21, Nº.22, 8622
    Abstract
    Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.
    Materias (normalizadas)
    Mitocondrias
    Genética médica
    Calcio - Metabolismo
    Materias Unesco
    2302.21 Biología Molecular
    Palabras Clave
    MCU
    ISSN
    1661-6596
    Revisión por pares
    SI
    DOI
    10.3390/ijms21228622
    Patrocinador
    Ministerio de Economía y Competitividad - (Proyecto BFU2017-83509-R)
    Fondo Europeo de Desarrollo Regional (FEDER) y Junta de Castilla y León - (Projecto VA011G18)
    Version del Editor
    https://www.mdpi.com/1422-0067/21/22/8622
    Propietario de los Derechos
    © 2020 The Authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/58458
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Collections
    • DEP06 - Artículos de revista [156]
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    Atribución 4.0 InternacionalExcept where otherwise noted, this item's license is described as Atribución 4.0 Internacional

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