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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/5951

    Título
    Ca 2+ induced Ca 2+ Release in Chromaffin Cells Seen from inside the ER with Targeted Aequorin
    Autor
    Alonso Alonso, María TeresaAutoridad UVA
    Barrero, María José
    Michelena, Pedro
    Carnicero Gila, Estela MaríaAutoridad UVA Orcid
    Cuchillo Ibáñez, Inmaculada
    García, Antonio G.
    García-Sancho Martín, Francisco JavierAutoridad UVA Orcid
    Montero Zoccola, María TeresaAutoridad UVA Orcid
    Álvarez Martín, JavierAutoridad UVA Orcid
    Año del Documento
    1999
    Editorial
    The Rockefeller University Press,
    Descripción
    Producción Científica
    Documento Fuente
    Journal of Cell Biology, Enero 1999, vol. 144, n. 2, p. 241-254
    Résumé
    The presence and physiological role of Ca 2+ induced Ca 2+ release (CICR) in nonmuscle excitable cells has been investigated only indirectly through measurements of cytosolic [Ca 2+] ([Ca 2+]c ). Using targeted aequorin, we have directly monitored [Ca 2+] changes inside the ER ([Ca 2+] ER ) in bovine adrenal chromaffin cells. Ca 2+ entry induced by cell depolarization triggered a transient Ca 2+release from the ER that was highly dependent on [Ca 2+] ER and sensitized by low concentrations of caffeine. Caffeine-induced Ca 2+ release was quantal in nature due to modulation by [Ca 2+] ER . Whereas caffeine released essentially all the Ca 2+ from the ER, inositol 1,4,5-trisphosphate (InsP3)- producing agonists released only 60Ð80%. Both InsP3 and caffeine emptied completely the ER in digitoninpermeabilized cells whereas cyclic ADP-ribose had no effect. Ryanodine induced permanent emptying of the Ca 2+ stores in a use-dependent manner after activation by caffeine. Fast confocal [Ca 2+]c measurements showed that the wave of [C 2+]c induced by 100-ms depolarizing pulses in voltage-clamped cells was delayed and reduced in intensity in ryanodine-treated cells. Our results indicate that the ER of chromaffin cells behaves mostly as a single homogeneous thapsigargin-sensitive Ca 2+ pool that can release Ca 2+ both via InsP3 receptors or CICR.
    Materias (normalizadas)
    Calcio - Metabolismo
    ISSN
    0021-9525
    Revisión por pares
    SI
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/5951
    Derechos
    openAccess
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    • DEP05 - Artículos de revista [198]
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    Alvarez 2- Ca 2+ induced Ca 2+.pdf
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    Attribution-NonCommercial-NoDerivatives 4.0 InternationalExcepté là où spécifié autrement, la license de ce document est décrite en tant que Attribution-NonCommercial-NoDerivatives 4.0 International

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