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dc.contributor.authorHernández San Miguel, Esther
dc.contributor.authorVay, Laura
dc.contributor.authorSanto Domingo, Jaime
dc.contributor.authorDomínguez Lobatón, María Carmen 
dc.contributor.authorMoreno Díaz-Calderón, Alfredo 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-15T12:01:52Z
dc.date.available2014-09-15T12:01:52Z
dc.date.issued2006
dc.identifier.citationCell Calcium, 2006, vol. 40, p. 53-61es
dc.identifier.issn0143-4160es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5952
dc.descriptionProducción Científicaes
dc.description.abstractThere is increasing evidence that mitochondria play an important role in the control of cytosolic Ca2+ signaling. We show here that the main mitochondrial Ca2+-exit pathway, the mitochondrial Na+/Ca2+ exchanger, controls the pattern of cytosolic Ca2+ oscillations in nonexcitable cells. In HeLa cells, the inhibitor of the mitochondrial Na+/Ca2+ exchanger CGP37157 changed the pattern of the oscillations induced by histamine from a high-frequency irregular one to a lower frequency baseline spike type, surprisingly with little changes in the average Ca2+ values of a large cell population. In human fibroblasts, CGP37157 increased the frequency of the baseline oscillations in cells having spontaneous activity and induced the generation of oscillations in cells without spontaneous activity. This effect was dose-dependent, disappeared when the inhibitor was washed out and was not mimicked by mitochondrial depolarization. CGP37157 increased mitochondrial [Ca2+] and ATP production in histamine-stimulated HeLa cells, but the effect on ATP production was only transient. CGP37157 also activated histamine-induced Ca2+ release from the endoplasmic reticulum and increased the size of the cytosolic Ca2+ peak induced by histamine in HeLa cells. Our results suggest that the mitochondrial Na+/Ca2+ exchanger directly modulates inositol 1,4,5-trisphosphate-induced Ca2+ release and in that way controls cytosolic Ca2+ oscillations.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevier Ltd.es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalcio - Metabolismoes
dc.titleThe mitochondrial Na+/Ca2+ exchanger plays a key role in the control of cytosolic Ca2+ oscillationses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/j.ceca.2006.03.009es
dc.identifier.publicationfirstpage53es
dc.identifier.publicationlastpage61es
dc.identifier.publicationtitleCell Calciumes
dc.identifier.publicationvolume40es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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