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dc.contributor.authorFonteriz García, Rosalba Inés 
dc.contributor.authorFuente Pérez, Sergio de la
dc.contributor.authorMoreno Díaz-Calderón, Alfredo 
dc.contributor.authorDomínguez Lobatón, María Carmen 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-15T16:50:12Z
dc.date.available2014-09-15T16:50:12Z
dc.date.issued2010
dc.identifier.citationCell Calcium, 2010, vol. 48, p. 61-69es
dc.identifier.issn0143-4160es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5959
dc.descriptionProducción Científicaes
dc.description.abstractThe dynamics of mitochondrial [Ca2+] ([Ca2+]M) plays a key role in a variety of cellular processes. The most important methods available to monitor [Ca2+]M are fluorescent dyes such as rhod-2 and specifically targeted proteins such as aequorin and pericam. However, significant discrepancies, both quantitative and qualitative, exist in the literature between the results obtained with different methods. We have made here a systematic comparison of the response of several fluorescent dyes, rhod-2 and rhod-FF, and two Ca2+-sensitive proteins, aequorin and pericam. Our results show that measurements obtained with aequorin and pericam are consistent in terms of dynamic Ca2+ changes. Instead, fluorescent dyes failed to follow Ca2+ changes adequately, especially during repetitive stimulation. In particular, measures obtained with rhod-2 or rhod-FF evidenced the previously reported Ca2+-dependent inhibition of mitochondrial Ca2+ uptake, but data obtained with aequorin or pericam under the same conditions did not. The reason for the loss of response of fluorescent dyes is unclear. Loading with these dyes produced changes in mitochondrial morphology and membrane potential, which were small and reversible at low concentrations (1–2 M), but produced large and prolonged damage at higher concentrations. In addition, cells loaded with low concentrations of rhod-2 suffered large changes in mitochondrial morphology after light excitation. Our results suggest that [Ca2+]M data obtained with these dyes should be taken with care.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevier Ltd.es
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalcio - Metabolismoes
dc.titleMonitoring mitochondrial [Ca2+] dynamics with rhod-2, ratiometric pericam and aequorines
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/j.ceca.2010.07.001es
dc.identifier.publicationfirstpage61es
dc.identifier.publicationlastpage69es
dc.identifier.publicationtitleCell Calciumes
dc.identifier.publicationvolume48es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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