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dc.contributor.authorSanto Domingo, Jaime
dc.contributor.authorVay, Laura
dc.contributor.authorCamacho, Marcial
dc.contributor.authorHernández San Miguel, Esther
dc.contributor.authorFonteriz García, Rosalba Inés 
dc.contributor.authorDomínguez Lobatón, María Carmen 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorMoreno Díaz-Calderón, Alfredo 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-16T09:52:10Z
dc.date.available2015-09-16T23:40:10Z
dc.date.issued2008
dc.identifier.citationEuropean Journal of Neuroscience, 2008, vol. 28, p. 1265-1274es
dc.identifier.issn0953-816Xes
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5977
dc.descriptionProducción Científicaes
dc.description.abstractThe secretory granules constitute one of the less well-known compartments in terms of Ca2+ dynamics. They contain large amounts of total Ca2+, but the free intragranular [Ca2+] ([Ca2+]SG), the mechanisms for Ca2+ uptake and release from the granules and their physiological significance regarding exocytosis are still matters of debate. We used in the present work an aequorin chimera targeted to the granules to investigate [Ca2+]SG homeostasis in bovine adrenal chromaffin cells. We found that most of the intracellular aequorin chimera is present in a compartment with 50–100 lm Ca2+. Ca2+ accumulation into this compartment takes place mainly through an ATP-dependent mechanism, namely, a thapsigargin-sensitive Ca2+-ATPase. In addition, fast Ca2+ release was observed in permeabilized cells after addition of inositol 1,4,5-trisphosphate (InsP3) or caffeine, suggesting the presence of InsP3 and ryanodine receptors in the vesicular membrane. Stimulation of intact cells with the InsP3-producing agonist histamine or with caffeine also induced Ca2+ release from the vesicles, whereas acetylcholine or high-[K+] depolarization induced biphasic changes in vesicular [Ca2+], suggesting heterogeneous responses of different vesicle populations, some of them releasing and some taking up Ca2+ during stimulation. In conclusion, our data show that chromaffin cell secretory granules have the machinery required for rapid uptake and release of Ca2+, and this strongly supports the hypothesis that granular Ca2+ may contribute to its own secretion.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherFederation of European Neuroscience Societies and Blackwell Publishing Ltdes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMédula espinal - Calcioes
dc.titleCalcium dynamics in bovine adrenal medulla chromaffin cell secretory granuleses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1111/j.1460-9568.2008.06440.xes
dc.identifier.publicationfirstpage1265es
dc.identifier.publicationlastpage1274es
dc.identifier.publicationtitleEuropean Journal of Neurosciencees
dc.identifier.publicationvolume28es
dc.peerreviewedSIes
dc.description.embargo2015-09-16es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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