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dc.contributor.authorSanto Domingo, Jaime
dc.contributor.authorFonteriz García, Rosalba Inés 
dc.contributor.authorDomínguez Lobatón, María Carmen 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorMoreno Díaz-Calderón, Alfredo 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-16T10:11:25Z
dc.date.available2014-09-16T10:11:25Z
dc.date.issued2010
dc.identifier.citationCellular and Molecular Neurobiology, 2010, vol. 30, p. 1267-1274es
dc.identifier.issn0272-4340es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5980
dc.descriptionProducción Científicaes
dc.description.abstractWe have investigated the dynamics of the free [Ca2+] inside the secretory granules of neurosecretory PC12 and INS1 cells using a low-Ca2+-affinity aequorin chimera fused to synaptobrevin-2. The steady-state secretory granule [Ca2+] ([Ca2+]SG] was around 20–40 lM in both cell types, about half the values previously found in chromaffin cells. Inhibition of SERCA-type Ca2+ pumps with thapsigargin largely blocked Ca2+ uptake by the granules in Ca2+-depleted permeabilized cells, and the same effect was obtained when the perfusion medium lacked ATP. Consistently, the SERCA-type Ca2+ pump inhibitor benzohydroquinone induced a rapid release of Ca2+ from the granules both in intact and permeabilized cells, suggesting that the continuous activity of SERCA-type Ca2+ pumps is essential to maintain the steady-state [Ca2+]SG. Both inositol 1,4, 5-trisphosphate (InsP3) and caffeine produced a rapid Ca2+ release from the granules, suggesting the presence of InsP3 and ryanodine receptors in the granules. The response to high-K+ depolarization was different in both cell types, a decrease in [Ca2+]SG in PC12 cells and an increase in [Ca2+]SG in INS1 cells. The difference may rely on the heterogeneous response of different vesicle populations in each cell type. Finally, increasing the glucose concentration triggered a decrease in [Ca2+]SG in INS1 cells. In conclusion, our data show that the secretory granules of PC12 and INS1 cells take up Ca2+ through SERCA-type Ca2+ pumps and can release it through InsP3 and ryanodine receptors, supporting the hypothesis that secretory granule Ca2+ may be released during cell stimulation and contribute to secretion.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherSpringer Verlages
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalcio - Metabolismoes
dc.titleCa2+ Dynamics in the Secretory Vesicles of Neurosecretory PC12 and INS1 Cellses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1007/s10571-010-9572-2es
dc.identifier.publicationfirstpage1267es
dc.identifier.publicationlastpage1274es
dc.identifier.publicationtitleCellular and Molecular Neurobiologyes
dc.identifier.publicationvolume30es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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