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dc.contributor.authorVay, Laura
dc.contributor.authorHernández San Miguel, Esther
dc.contributor.authorSanto Domingo, Jaime
dc.contributor.authorDomínguez Lobatón, María Carmen 
dc.contributor.authorMoreno Díaz-Calderón, Alfredo 
dc.contributor.authorMontero Zoccola, María Teresa 
dc.contributor.authorÁlvarez Martín, Javier 
dc.date.accessioned2014-09-16T10:59:24Z
dc.date.available2014-09-16T10:59:24Z
dc.date.issued2007
dc.identifier.citationJournal of Physiology, 2007, vol. 580, n. 1, p. 39-49es
dc.identifier.issn0022-3751es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/5984
dc.descriptionProducción Científicaes
dc.description.abstractThe recent availability of activators of the mitochondrial Ca2+ uniporter allows direct testing of the influence of mitochondrial Ca2+ uptake on the overall Ca2+ homeostasis of the cell. We show here that activation of mitochondrial Ca2+ uptake by 4,4 ,4 -(4- propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT) or kaempferol stimulates histamine-induced Ca2+ release from the endoplasmic reticulum (ER) and that this effect is enhanced if the mitochondrial Na+–Ca2+ exchanger is simultaneously inhibited with CGP37157. This suggests that both Ca2+ uptake and release from mitochondria control the ability of local Ca2+ microdomains to produce feedback inhibition of inositol 1,4,5-trisphosphate receptors (InsP3Rs). In addition, the abilityof mitochondria tocontrolCa2+ release fromtheERallowsthemtomodulate cytosolic Ca2+ oscillations. In histamine stimulatedHeLa cells and human fibroblasts, both PPT and kaempferol initially stimulated and later inhibited oscillations, although kaempferol usually induced a more prolonged period of stimulation. Both compounds were also able to induce the generation of Ca2+ oscillations in previously silent fibroblasts. Our data suggest that cytosolic Ca2+ oscillations are exquisitely sensitive to the rates of mitochondrial Ca2+ uptake and release, which precisely control the size of the local Ca2+ microdomains around InsP3Rs and thus the ability to produce feedback activation or inhibition of Ca2+ release.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCalcio en el organismoes
dc.titleModulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulationes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1113/jphysiol.2006.126391es
dc.identifier.publicationfirstpage39es
dc.identifier.publicationissue1es
dc.identifier.publicationlastpage49es
dc.identifier.publicationtitleJournal of Physiologyes
dc.identifier.publicationvolume580es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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