Protective effects of filtrates and extracts from fungal endophytes on Phytophthora cinnamomi in Lupinus luteus

Abstract

Fungal endophytes have been found to protect their hosts against multiple fungal pathogens. Frequently, the secondary metabolites produced by the endophyte are responsible for antifungal activity. To develop new bio-products that are more environmentally friendly than synthetic pesticides against Phytophthora cinnamomi, a serious pathogen of many plant species, the antifungal activity of filtrates or extracts from four endophytes was evaluated in different in vitro tests and in plants of Lupinus luteus. In the dual culture assays, the filtrate of one of the endophytes (Drechslera biseptata) completely inhibited the mycelial growth of the pathogen. Moreover, it showed a very low minimal inhibitory concentration (MIC). Epicoccum nigrum, an endophyte that also showed high inhibitory activity and a low MIC against P. cinnamomi in those two experiments, provided a clear growth promotion effect when the extracts were applied to L. luteus seedlings. The extract of Fusarium avenaceum also manifested such a promotion effect and was the most effective in reducing the disease severity caused by the pathogen in lupine plants (73% reduction). Results demonstrated the inhibitory activity of the filtrates or extracts of these endophytes against P. cinnamomi. A better insight into the mechanisms involved may be gained by isolating and identifying the metabolites conferring this inhibitory effect against this oomycete pathogen.