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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/61984

    Título
    Modulation of multiple gene clusters’ expression by the PAS-LuxR transcriptional regulator PteF
    Autor
    Vicente, Cláudia
    Payero, Tamara
    Rodríguez García, Antonio
    Barreales, Eva
    Pedro, Antonio de
    Santos Beneit, FernandoAutoridad UVA Orcid
    Aparicio, Jesús F.
    Año del Documento
    2022
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Antibiotics, 2022, Vol. 11, Nº. 8, 994
    Resumen
    PAS-LuxR transcriptional regulators are conserved proteins governing polyene antifungal biosynthesis. PteF is the regulator of filipin biosynthesis from Streptomyces avermitilis. Its mutation drastically abates filipin, but also oligomycin production, a macrolide ATP-synthase inhibitor, and delays sporulation; thus, it has been considered a transcriptional activator. Transcriptomic analyses were performed in S. avermitilis ΔpteF and its parental strain. Both strains were grown in a YEME medium without sucrose, and the samples were taken at exponential and stationary growth phases. A total of 257 genes showed an altered expression in the mutant, most of them at the exponential growth phase. Surprisingly, despite PteF being considered an activator, most of the genes affected showed overexpression, thereby suggesting a negative modulation. The affected genes were related to various metabolic processes, including genetic information processing; DNA, energy, carbohydrate, and lipid metabolism; morphological differentiation; and transcriptional regulation, among others, but were particularly related to secondary metabolite biosynthesis. Notably, 10 secondary metabolite gene clusters out of the 38 encoded by the genome showed altered expression profiles in the mutant, suggesting a regulatory role for PteF that is wider than expected. The transcriptomic results were validated by quantitative reverse-transcription polymerase chain reaction. These findings provide important clues to understanding the intertwined regulatory machinery that modulates antibiotic biosynthesis in Streptomyces.
    Materias (normalizadas)
    Antifungal agents
    Genetic regulation
    Regulación genética
    Medical genetics
    Antibiotics
    Antibióticos
    Streptomyces
    Genética - Bacterias
    Materias Unesco
    3303 Ingeniería y Tecnología Químicas
    3201.02 Genética Clínica
    ISSN
    2079-6382
    Revisión por pares
    SI
    DOI
    10.3390/antibiotics11080994
    Patrocinador
    Ministerio de Economía, Industria y Competitividad - (grants BIO2013-42983-P and PCIN-2016-190)
    Ministerio de Educación, Cultura y Deporte - (Projects AP2007-02055 and FPU13/01537)
    Fundación Portuguesa para la Ciencia y la Tecnología - (grant SFRH/BD/64006/2009)
    Version del Editor
    https://www.mdpi.com/2079-6382/11/8/994
    Propietario de los Derechos
    © 2022 The Authors
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/61984
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • DEP48 - Artículos de revista [265]
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    Modulation-of-Multiple-Gene-Clusters’-Expression.pdf
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    Universidad de Valladolid

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