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Título
The coupling of plasma membrane calcium entry to calcium uptake by endoplasmic reticulum and mitochondria
Año del Documento
2014
Editorial
The Physiological Society
Descripción
Producción Científica
Documento Fuente
Journal of Physiology, 2014, p. 261-268
Résumé
Cross-talk between organelles and plasma membrane Ca2+ channels is essential for
modulation of the cytosolic Ca2+ ([Ca2+]C) signals, but such modulation may differ among
cells. In chromaffin cells Ca2+ entry through voltage-operated channels induces calcium release
from the endoplasmic reticulum (ER) that amplifies the signal. [Ca2+]C microdomains as high
as 20–50 μM are sensed by subplasmalemmal mitochondria, which accumulate large amounts of
Ca2+ through the mitochondrial Ca2+ uniporter (MCU). Mitochondria confine the high-Ca2+
microdomains (HCMDs) to beneath the plasma membrane,where exocytosis of secretory vesicles
happens. Cell core [Ca2+]C is much smaller (1–2 μM). By acting as a Ca2+ sink, mitochondria
stabilise theHCMDin space and time. In non-excitableHEK293 cells, activation of store-operated
Ca2+ entry, triggered by ERCa2+ emptying, also generated subplasmalemmal HCMDs, but, in this
case, most of the Ca2+ was taken up by the ER rather than bymitochondria. The smaller size of the
[Ca2+]C peak in this case (about 2 μM)may contribute to this outcome, as the sarco-endoplasmic
reticulum Ca2+ ATPase has much higher Ca2+ affinity than MCU. It is also possible that the relative positioning of organelles, channels and effectors, as well as cytoskeleton and accessory
proteins plays an important role.
Materias (normalizadas)
Calcio - Absorción
ISSN
0022-3751
Revisión por pares
SI
Idioma
eng
Derechos
openAccess
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