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Título
Functionalization of 3D-printed titanium scaffolds with elastin-like recombinamers to improve cell colonization and osteoinduction
Autor
Año del Documento
2023
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Pharmaceutics, 2023, Vol. 15, Nº. 3, 872
Résumé
The 3D printing of titanium (Ti) offers countless possibilities for the development of personalized implants with suitable mechanical properties for different medical applications. However, the poor bioactivity of Ti is still a challenge that needs to be addressed to promote scaffold osseointegration. The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. To this end, ELRs containing specific cell-adhesive (RGD) and/or osteoinductive (SNA15) moieties were covalently attached to Ti scaffolds. Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. These results suggest that biofunctionalization with SNA15-ELRs could modulate the cellular response to improve the osseointegration of Ti implants. Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study.
Materias (normalizadas)
3D Printing
Three-dimensional printing
Impresión tridimensional
Imágenes tridimensionales en medicina
Diseño asistido por ordenador
Polymers in medicine
Polímeros recombinantes
Polímeros - Aplicaciones médicas
Biomaterials
Biomateriales
Osseointegration
Titanium
Pharmaceutical technology
Pharmacology
Materias Unesco
2206.10 Polímeros
2415 Biología Molecular
3209 Farmacología
3312 Tecnología de Materiales
Palabras Clave
Functionalization
ISSN
1999-4923
Revisión por pares
SI
Patrocinador
Ministerio de Ciencia e Innovación y Fondo Europeo de Desarrollo Regional (FEDER) - (projects PID2021-125150OB-I00, DTS19/00162, and PID2019-106386RB-I00)
Version del Editor
Propietario de los Derechos
© 2023 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Fichier(s) constituant ce document
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