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dc.contributor.authorWei, Qianru
dc.contributor.authorBrzostek, Joanna
dc.contributor.authorSankaran, Shvetha
dc.contributor.authorCasas, Javier
dc.contributor.authorHew, Lois Shi-Qi
dc.contributor.authorYap, Jiawei
dc.contributor.authorZhao, Xiang
dc.contributor.authorWojciech, Lukasz
dc.contributor.authorGascoigne, Nicholas R. J.
dc.date.accessioned2024-01-08T14:38:29Z
dc.date.available2024-01-08T14:38:29Z
dc.date.issued2020
dc.identifier.citationP Natl Acad Sci Usa 201913334. https://doi.org/10.1073/pnas.1913334117es
dc.identifier.issn0027-8424es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/64304
dc.descriptionProducción Científicaes
dc.description.abstractSrc family kinase Lck plays critical roles during T cell development and activation, as it phosphorylates the TCR/CD3 complex to initiate TCR signaling. Lck is present either in coreceptor-bound or coreceptor-unbound (free) forms, and we here present evidence that the two pools of Lck have different molecular properties. We discovered that the free Lck fraction exhibited higher mobility than CD8α-bound Lck in OT-I T hybridoma cells. The free Lck pool showed more activating Y394 phosphorylation than the coreceptor-bound Lck pool. Consistent with this, free Lck also had higher kinase activity, and free Lck mediated higher T cell activation as compared to coreceptor-bound Lck. Furthermore, the coreceptor-Lck coupling was independent of TCR activation. These findings give insights into the initiation of TCR signaling, suggesting that changes in coreceptor-Lck coupling constitute a mechanism for regulation of T cell sensitivity.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0*
dc.titleLck bound to coreceptor is less active than free Lckes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1073/pnas.1913334117es
dc.identifier.publicationfirstpage15809es
dc.identifier.publicationissue27es
dc.identifier.publicationlastpage15817es
dc.identifier.publicationtitleProceedings of the National Academy of Scienceses
dc.identifier.publicationvolume117es
dc.peerreviewedSIes
dc.description.projectMinisterio de Singapur de Salud del Consejo Nacional de Investigaciones Médicas mediante su CBRG/0097/2015 y otorgado por la subvención 2014-T2-1-136 del Ministerio de Educación de Singapur.
dc.identifier.essn1091-6490es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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