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dc.contributor.author | Wei, Qianru | |
dc.contributor.author | Brzostek, Joanna | |
dc.contributor.author | Sankaran, Shvetha | |
dc.contributor.author | Casas, Javier | |
dc.contributor.author | Hew, Lois Shi-Qi | |
dc.contributor.author | Yap, Jiawei | |
dc.contributor.author | Zhao, Xiang | |
dc.contributor.author | Wojciech, Lukasz | |
dc.contributor.author | Gascoigne, Nicholas R. J. | |
dc.date.accessioned | 2024-01-08T14:38:29Z | |
dc.date.available | 2024-01-08T14:38:29Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | P Natl Acad Sci Usa 201913334. https://doi.org/10.1073/pnas.1913334117 | es |
dc.identifier.issn | 0027-8424 | es |
dc.identifier.uri | https://uvadoc.uva.es/handle/10324/64304 | |
dc.description | Producción Científica | es |
dc.description.abstract | Src family kinase Lck plays critical roles during T cell development and activation, as it phosphorylates the TCR/CD3 complex to initiate TCR signaling. Lck is present either in coreceptor-bound or coreceptor-unbound (free) forms, and we here present evidence that the two pools of Lck have different molecular properties. We discovered that the free Lck fraction exhibited higher mobility than CD8α-bound Lck in OT-I T hybridoma cells. The free Lck pool showed more activating Y394 phosphorylation than the coreceptor-bound Lck pool. Consistent with this, free Lck also had higher kinase activity, and free Lck mediated higher T cell activation as compared to coreceptor-bound Lck. Furthermore, the coreceptor-Lck coupling was independent of TCR activation. These findings give insights into the initiation of TCR signaling, suggesting that changes in coreceptor-Lck coupling constitute a mechanism for regulation of T cell sensitivity. | es |
dc.format.mimetype | application/pdf | es |
dc.language.iso | eng | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0 | * |
dc.title | Lck bound to coreceptor is less active than free Lck | es |
dc.type | info:eu-repo/semantics/article | es |
dc.identifier.doi | 10.1073/pnas.1913334117 | es |
dc.identifier.publicationfirstpage | 15809 | es |
dc.identifier.publicationissue | 27 | es |
dc.identifier.publicationlastpage | 15817 | es |
dc.identifier.publicationtitle | Proceedings of the National Academy of Sciences | es |
dc.identifier.publicationvolume | 117 | es |
dc.peerreviewed | SI | es |
dc.description.project | Ministerio de Singapur de Salud del Consejo Nacional de Investigaciones Médicas mediante su CBRG/0097/2015 y otorgado por la subvención 2014-T2-1-136 del Ministerio de Educación de Singapur. | |
dc.identifier.essn | 1091-6490 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es |
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