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dc.contributor.authorDrum, Benjamin M.
dc.contributor.authorYuan, Can
dc.contributor.authorDe La Mata Sampedro, Ana 
dc.contributor.authorGrainger, Nathan
dc.contributor.authorSantana, L. Fernando
dc.date.accessioned2024-02-01T12:49:23Z
dc.date.available2024-02-01T12:49:23Z
dc.date.issued2020
dc.identifier.citationBenjamin M.Drum, Can Yuan, Ana de la Mata, Nathan Grainger, L. Fernando Santana. Junctional sarcoplasmic reticulum motility in adult mouse ventricular myocytes. American Journal of Physiology_Cell Physiology. 2020. 318:598-604.es
dc.identifier.issn0363-6143es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/65511
dc.description.abstractExcitation-contraction (EC) coupling is the coordinated process by which an action potential triggers cardiac myocyte contraction. EC coupling is initiated in dyads where the junctional sarcoplasmic reticulum (jSR) is in tight proximity to the sarcolemma of cardiac myocytes. Existing models of EC coupling critically depend on dyad stability to ensure the fidelity and strength of EC coupling, where even small variations in ryanodine receptor channel and voltage-gated calcium channel-α 1.2 subunit separation dramatically alter EC coupling. However, dyadic motility has never been studied. Here, we developed a novel strategy to track specific jSR units in dissociated adult ventricular myocytes using photoactivatable fluorescent proteins. We found that the jSR is not static. Instead, we observed dynamic formation and dissolution of multiple dyadic junctions regulated by the microtubule-associated molecular motors kinesin-1 and dynein. Our data support a model where reproducibility of EC coupling results from the activation of a temporally averaged number of SR Ca2+ release units forming and dissolving SR-sarcolemmal junctions. These findings challenge the long-held view that the jSR is an immobile structure and provide insights into the mechanisms underlying its motility.es
dc.format.mimetypeapplication/pdfes
dc.language.isospaes
dc.publisherAMER PHYSIOLOGICAL SOCes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/*
dc.titleJunctional sarcoplasmic reticulum motility in adult mouse ventricular myocyteses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1152/ajpcell.00573.2019es
dc.identifier.publicationfirstpageC598es
dc.identifier.publicationissue3es
dc.identifier.publicationlastpageC604es
dc.identifier.publicationtitleAmerican Journal of Physiology-Cell Physiologyes
dc.identifier.publicationvolume318es
dc.peerreviewedSIes
dc.identifier.essn1522-1563es
dc.rightsCC0 1.0 Universal*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones


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