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dc.contributor.authorDomínguez-López, Alfredo
dc.contributor.authorBlanco Vázquez, Marta 
dc.contributor.authorCalderón García, Andrés Ángel
dc.contributor.authorGarcía-Vázquez, Carmen
dc.contributor.authorGonzález García, María Jesús 
dc.contributor.authorCalonge, Margarita 
dc.contributor.authorEnriquez De Salamanca Aladro, Amalia 
dc.date.accessioned2024-04-05T09:43:51Z
dc.date.available2024-04-05T09:43:51Z
dc.date.issued2024
dc.identifier.citationExp Eye Res. Abril 2024, vol 241:109854es
dc.identifier.issn00144835es
dc.identifier.urihttps://uvadoc.uva.es/handle/10324/67033
dc.descriptionProducción Científicaes
dc.description.abstractMucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under in vitro dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients. Human corneal epithelial cells (HCE) and immortalized human conjunctival epithelial cells (IM-ConEpiC) were incubated under hyperosmolar (400–500 mOsM) or inflammatory (TNF-α 25 ng/mL) conditions for 6 h and 24 h to measure CCL28, CXCL14, and CXCL17 gene expression by RT-PCR and their secretion by immunobead-based analysis (CCL28, CXCL14) and ELISA (CXCL17). Additionally, twenty-seven DE patients and 13 healthy subjects were included in this study. DE-related questionnaires (OSDI, mSIDEQ and NRS) evaluated symptomatology. Ocular surface integrity was assessed using vital staining. Tactile sensitivity was measured with Cochet-Bonnet esthesiometer, and mechanic and thermal (heat and cold) sensitivity using Belmonte’s non-contact esthesiometer. Subbasal nerve plexus and dendritic cell density were analyzed by in vivo confocal microscopy. Conjunctival cells from participants were collected by impression cytology to measure mucosal chemokines gene expression by RTPCR. Our results showed that HCE and IM-HConEpiC cells increased CCL28, CXCL14, and CXCL17 secretion under hyperosmolar conditions. The gene expression of CCL28 was significantly upregulated in conjunctival samples from DE patients. CCL28 expression correlated positively with symptomatology, corneal staining, heat sensitivity threshold, and dendritic cell density. CXCL14 expression correlated positively with age, ocular pain, conjunctival staining, tactile sensitivity, and image reflectivity. CXCL17 expression correlated positively with corneal staining. These results suggest that corneal and conjunctival epithelial cells could be a source of CCL28, CXCL14, and CXCL17 on the ocular surface and that CCL28 might be involved in DE pathogenesis.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationOcular surface Dry eye, Mucosal chemokines ,Hyperosmolarity, Inflammation, CCL28, CXCL14, CXCL17es
dc.titleAnalysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigationes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1016/j.exer.2024.109854es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0014483524000757?via%3Dihubes
dc.identifier.publicationtitleAnalysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigationes
dc.identifier.publicationvolume241es
dc.peerreviewedSIes
dc.description.projectEste trabajo forma parte de los proyectos de investigación: SAF-2016-77080-P, financiado por MICIU/AEI /10.13039/501100011033/ y por FEDER Una manera de hacer Europa; por proyecto PID 2022-142578OB-I00, financiado por MICIU/AEI/10.13039/501100011033 y por FEDER, UE; y por grant SECTEI/160/ 2021 fianciado por Ministerio de Educacion, Ciencia, Tecnología e Innovación de Ciudad de Méjico (SECTEI).es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones
dc.subject.unesco2403 - BIOQUIMICA, 2410 - BIOLOGIA HUMANA, 2412 - INMUNOLOGIA 2415, - BIOLOGIA MOLECULAR, 3299 - OTRAS ESPECIALIDADES MEDICASes


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