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dc.contributor.authorDinger, Bruce
dc.contributor.authorAlmaraz Gómez, Laura
dc.contributor.authorHirano, T.
dc.contributor.authorYoshizaki, Katsuaki
dc.contributor.authorGonzález, Constancio
dc.contributor.authorGómez Niño, María Ángeles 
dc.contributor.authorFidone, Salvatore
dc.date.accessioned2014-10-30T12:34:34Z
dc.date.available2014-10-30T12:34:34Z
dc.date.issued1991
dc.identifier.citationBrain Research, 1991, vol.562, p.190-198es
dc.identifier.issn0006-8993es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/6843
dc.descriptionProducción Científicaes
dc.description.abstractAcetylcholine and muscarinic agonists inhibit chemosensory activity in the rabbit carotid sinus nerve (CSN). Because the mechanism of this inhibition is poorly understood, we have investigated the kinetics and distribution of muscarinic receptors in the rabbit carotid body with the specific muscarinic antagonist [SH]quinuclidinylbenzitate ([3H]QNB). Equilibrium binding experiments identified displaceable binding sites (1/~M atropine) with a K d = 71.46 pM and a Bm~ x = 9.23 pmol/g tissue. These binding parameters and the pharmacology of the displaceable [SH]QNB binding sites are similar to specific muscannic receptors identified in numerous other nervous, muscular and glandular tissues. Comparisons of specific binding in normal and chronic CSN-denervated carotid bodies suggest that musearinic receptors are absent on afferent terminals in the carotid body; however, nearly 50% of the specific [3H]QNB binding is lost following chronic sympathectomy, suggesting the presence of presynaptic muscarinic receptors on the sympathetic innervation supplying the carotid body vasculature. Autoradiographic studies have localized the remainder of [3H]QNB binding sites to Iobules of type I and type II parenchymal cells. In separate experiments, the muscarinic agonists, oxotremorine (100/~M) and bethanechol (100 ~tM) were shown to inhibit both the release of catecholamines and the increased CSN activity evoked by nicotine (50/~M) stimulation of the in vitro carotid body, Our data suggest that muscarinic inhibition in the rabbit carotid body is mediated by receptors located on type I cells which are able to modulate the excitatory actions of acetylcholine at nicotinic sites.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNeurofisiologíaes
dc.titleMuscarinic receptor localization and function in rabbit carotid bodyes
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/0006-8993(91)90621-2
dc.identifier.publicationfirstpage190es
dc.identifier.publicationlastpage198es
dc.identifier.publicationtitleBrain Researches
dc.identifier.publicationvolume562es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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