Features of scots pine mortality due to incursion of pine bark beetles in symbiosis with ophiostomatoid fungi in the forest-steppe of central Siberia

Abstract

Forest decline is a significant issue affecting critical ecosystem processes worldwide. Here, we describe mortality in Pinus sylvestris L. monitored trees caused by the inhabitation of pine bark beetles (Tomicus minor Hart.) in symbiosis with ophiostomatoid fungi (Ophiostoma piceae (Munch) H. et P. Sydow) infection in the forest-steppe of central Siberia. Stem sap flow (Q) and stem diameter fluctuations (dRc) were monitored in eight pine trees during seven consecutive growing seasons (2015–2021). In addition, microcore sampling every ten days allowed the determination of stem wood formation in monitored trees in the 2021 growing season. During 2020 and 2021, two cases of Q termination were recorded among the monitored trees, with microcores revealing no cambium formation. Thus, the seasonal Q onset matches the beginning of the beetle dispersal period when they attack and inhabit tree stems. The decline of circumferential stem size began 10–12 days after Q onset, during the massive inhabitation of beetles into the stems. The disturbance of Q in trees occurred in 21–23 days, and total cessation of Q was observed 23–26 days after the Q onset at the beetle’s egg development phase. The timing of dRc disturbance and Q cessation observed directly coincides with the beetle life cycle. Thus, the phenology of pine trees and T. minor beetles is driven by seasonal weather conditions, particularly the cumulative air temperature (>0 °C).