Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/69397
Título
Characterization of the antitumor potential of extracts of Cannabis sativa strains with high CBD content in human neuroblastoma
Autor
Año del Documento
2023
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
International Journal of Molecular Sciences, 2023, Vol. 24, Nº. 4, 3837
Resumen
Cannabis has been used for decades as a palliative therapy in the treatment of cancer. This is because of its beneficial effects on the pain and nausea that patients can experience as a result of chemo/radiotherapy. Tetrahydrocannabinol and cannabidiol are the main compounds present in Cannabis sativa, and both exert their actions through a receptor-mediated mechanism and through a non-receptor-mediated mechanism, which modulates the formation of reactive oxygen species. These oxidative stress conditions might trigger lipidic changes, which would compromise cell membrane stability and viability. In this sense, numerous pieces of evidence describe a potential antitumor effect of cannabinoid compounds in different types of cancer, although controversial results limit their implementation. In order to further investigate the possible mechanism involved in the antitumoral effects of cannabinoids, three extracts isolated from Cannabis sativa strains with high cannabidiol content were analyzed. Cell mortality, cytochrome c oxidase activity and the lipid composition of SH-SY5Y cells were determined in the absence and presence of specific cannabinoid ligands, with and without antioxidant pre-treatment. The cell mortality induced by the extracts in this study appeared to be related to the inhibition of the cytochrome c oxidase activity and to the THC concentration. This effect on cell viability was similar to that observed with the cannabinoid agonist WIN55,212-2. The effect was partially blocked by the selective CB1 antagonist AM281, and the antioxidant α-tocopherol. Moreover, certain membrane lipids were affected by the extracts, which demonstrated the importance of oxidative stress in the potential antitumoral effects of cannabinoids.
Materias (normalizadas)
Cannabis
Cannabis - Therapeutic use
Cannabis - Uso terapeútico
Cannabinoids
Plant extracts
Drugs
Drogas
Medicinal plants
Plantas medicinales
Pharmacology
Neuroblastoma
Cancer
Cancer - Traitement
Cáncer - Tratamiento
Oncology
Antitumor
Materias Unesco
3209 Farmacología
2302.21 Biología Molecular
3201.01 Oncología
ISSN
1422-0067
Revisión por pares
SI
Patrocinador
Ministerio de Economía y Competitividad - (grants DIN2019-010902 and DIN2020-011349 )
Gobierno Vasco, Departamento de Desarrollo Económico, Sostenibilidad y Medio Ambiente - (Bikaintek program: 005-B2/2021)
Gobierno Vasco, Departamento de Desarrollo Económico, Sostenibilidad y Medio Ambiente - (Bikaintek program: 005-B2/2021)
Version del Editor
Propietario de los Derechos
© 2023 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
Aparece en las colecciones
Ficheros en el ítem
Tamaño:
674.1Kb
Formato:
Adobe PDF
La licencia del ítem se describe como Atribución 4.0 Internacional