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Título
A coating based on bioactive compounds from Streptomyces spp. and chitosan oligomers to control Botrytis cinerea preserves the quality and Improves the shelf life of table grapes
Autor
Año del Documento
2023
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Plants, 2023, Vol. 12, Nº. 3, 577
Resumen
Botrytis cinerea is the most harmful postharvest disease of table grapes. Among the strategies that can be envisaged for its control, the use of coatings based on natural products is particularly promising. The study presented herein focuses on the assessment of the antagonistic capacity of two Streptomyces species and their culture filtrates against B. cinerea. Firstly, the secondary metabolites were characterized by gas chromatography–mass spectrometry, with N1-(4-hydroxybutyl)-N3-methylguanidine acetate and 2R,3S-9-[1,3,4-trihydroxy-2-butoxymethyl]guanine acetate as the main compounds produced by S. lavendofoliae DSM 40217; and cyclo(leucyloprolyl) and cyclo(phenylalanylprolyl) as the most abundant chemical species for S. rochei DSM 41729. Subsequently, the capacity of S. lavendofoliae DSM 40217 and S. rochei DSM 41729 to inhibit the growth of the pathogen was tested in dual culture plate assays, finding 85–90% inhibition. In agar dilution tests, their culture filtrates resulted in effective concentration values (EC90) in the 246–3013 μg·mL−1 range. Upon the formation of conjugate complexes with chitosan oligomers (COS) to improve solubility and bioavailability, a synergistic behavior was observed, resulting in lower EC90 values, ranging from 201 to 953 μg·mL−1. Ex situ tests carried out on ‘Timpson’ and ‘Red Globe’ table grapes using the conjugate complexes as coatings were found to maintain the turgor of the grapes and delay the appearance of the pathogen by 10−15 days at concentrations in the 750−1000 µg·mL−1 range. Hence, the conjugate complexes of COS and the selected Streptomyces spp. culture filtrates may be put forward as promising protection treatments for the sustainable control of gray mold.
Materias (normalizadas)
Botrytis cinerea
Fungi
Plant diseases
Plantas - Enfermedades y plagas
Fruit - Postharvest diseases and injuries
Frutas - Enfermedades y plagas
Phytopathologie
Synergism
Antifungal
Fungicides
Table grapes
Uvas - Variedades
Uvas - Enfermedades y plagas
Grapes - Diseases and pests
Ecology
Plant science
Materias Unesco
2414.06 Hongos
3108.01 Bacterias
3108 Fitopatología
3101.05 Fungicidas
2417.13 Ecología Vegetal
3309 Tecnología de Los Alimentos
ISSN
2223-7747
Revisión por pares
SI
Version del Editor
Propietario de los Derechos
© 2023 The authors
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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La licencia del ítem se describe como Atribución 4.0 Internacional