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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/74262

    Título
    Functional effects of polyamines via activation of human β1- and β2 -adrenoceptors stably expressed in CHO cells
    Autor
    Meana González, ClaraAutoridad UVA Orcid
    Bordallo, Javier
    Bordallo, Carmen
    Suárez, Lorena
    Cantabrana, Begoña
    Sánchez, Manuel
    Año del Documento
    2010
    Documento Fuente
    Pharmacological Reports, julio 2010, vol. 62, n.4, p. 696-706
    Resumen
    Polyamines mediate acute metabolic effects and cardiac hypertrophy associated with β-adrenoceptor stimulation. They may also modulate β-adrenoceptors, causing functional responses in rat atria and tracheal smooth muscle. The aim of this study was to determine whether polyamines interact with human β(1)- and β(2)-adrenoceptors and the functional consequences of such an interaction. Chinese hamster ovary (CHO) cells stably transfected with human β(1)- and β(2)-adrenoceptors were used to evaluate the effect of polyamines binding to β-adrenoceptors, cAMP production and morphological changes, which were pharmacologically validated by investigating the effects of the β-adrenoceptor agonists, isoproterenol and salbutamol. Polyamines interacted with human β(1)- and β(2)-adrenoceptors, as shown by the displacement of [(125)I]iodocyanopindolol in the binding assay. Putrescine showed higher affinity to β(1)- than β(2)-adrenoceptors. Spermidine and spermine produced partial displacement (approximately 50%) and, at the highest concentration, the effect was reversed. Putrescine and spermine acutely increased cAMP and, in a serum-free medium, induced a stellate-like form in cells, which was inhibited by propranolol, a β-blocker. A 10 to 15 h incubation with putrescine produced a spindle-like form and spatial organization via β-adrenoceptor activation, evidenced by the antagonizing effect by propranolol and lack of effect in wild-type CHO cells. Additionally, it decreased cell proliferation independently of β-adrenoceptor activation. Spermine caused cell death via fetal bovine serum-dependent and -independent mechanisms. The results suggest that putrescine may act as a non-selective and low affinity agonist of human β(1)- and β(2)-adrenoceptors, eliciting morphological changes. These findings may be of importance in physiology and in diseases involving β-adrenoceptor functionality.
    ISSN
    1734-1140
    Revisión por pares
    SI
    DOI
    10.1016/s1734-1140(10)70327-3
    Version del Editor
    https://www.sciencedirect.com/science/article/abs/pii/S1734114010703273
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/74262
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
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    • DEP55 - Artículos de revista [206]
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    Nombre:
    04. 2010, PR. Functional effects of polyamines via activation of human b1 and b2-adrenoreceptors stably expressed in CHO cells.pdf
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