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    Por favor, use este identificador para citar o enlazar este ítem:https://uvadoc.uva.es/handle/10324/74270

    Título
    Subcellular Localization and Role of Lipin-1 in Human Macrophages
    Autor
    Valdearcos, Martín
    Esquinas, Esperanza
    Meana González, ClaraAutoridad UVA Orcid
    Gil de Gómez, Luis
    Guijas, Carlos
    Balsinde Rodríguez, Jesús
    Balboa García, María Ángeles
    Año del Documento
    2011
    Documento Fuente
    Journal of Immunology, mayo 2011, vol. 186, n. 10, p. 6004-6013
    Zusammenfassung
    The lipins have been described as metabolic enzymes that regulate lipid biosynthesis and also signaling processes by controlling the cellular concentration of bioactive lipids, phosphatidic acid, and diacylgycerol. In the present work we have studied the subcellular localization and role of lipin-1 in human monocyte-derived macrophages. Human macrophages express lipin-1 isoforms a and b. A transfected lipin-1a–enhanced GFP construct associates with membranes of cellular organelles that can be stained with Nile Red. Colocalization experiments with lipid droplet (LD)-specific proteins such as adipophilin/adipose differentiation-related protein/perilipin 2 or TIP47/perilipin 3 show that both proteins colocalize with lipin-1a in the same cellular structures. Reduction of the expression levels of lipin-1 by small interfering RNA technology does not impair triacylglycerol biosynthesis but reduces the size of LDs formed in response to oleic acid. In agreement with these data, peritoneal macrophages from animals that carry a mutation in the Lpin-1 gene (fld animals) also produce less and smaller LDs in response to oleic acid. Mass spectrometry determinations demonstrate that the fatty acid composition of triacylglycerol in isolated LDs from lipin-1–deficient cells differs from that of control cells. Moreover, activation of cytosolic group IVA phospholipase A2a, a proinflammatory enzyme that is also involved in LD biogenesis, is also compromised in lipin-1–deficient cells. Collectively, these data suggest that lipin-1 associates with LDs and regulates the activation of cytosolic group IVA phospholipase A2a in human monocyte-derived macrophages.
    ISSN
    0022-1767
    Revisión por pares
    SI
    DOI
    10.4049/jimmunol.1003279
    Idioma
    eng
    URI
    https://uvadoc.uva.es/handle/10324/74270
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP55 - Artículos de revista [206]
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    Nombre:
    09. 2011, JI. Subcellular localization and role of Lipin-1 in human MQ.pdf
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