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Título
Chitin and chitosan quantification in fungal cell wall via Raman spectroscopy
Autor
Año del Documento
2025
Editorial
Elsevier
Descripción
Producción Científica
Documento Fuente
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2025, vol. 334, p. 125928
Resumen
Investigation of cell wall composition is necessary to understand the interactions between fungi and the environment as it is the external layer exposed to stimuli and detected by other organisms. Pochonia chlamydosporia and Akanthomyces lecanii, two fungal species living in the soil and infecting nematodes and insects, exhibit endophytic interactions with various plant species. Determination of cell wall composition is essential to understand the mechanisms underlying these interactions. Therefore, in this study, for the first time, we assessed the relative amounts of chitin and chitosan in the cell walls of P. chlamydosporia (PC123) and A. lecanii (69NZ, 85SCT, 126KNY, and 447SAF) via Raman spectroscopy. The isolate with the highest chitosan percentage was 69NZ, followed by 85SCT, PC123, 447SAF, and 126KNY. Moreover, combination with conventional approaches for chitin and chitosan quantification yielded quantitative results for all cell wall components. Overall, these results highlight the mechanisms by which fungi exhibit chitosan resistance and avoid detection by the host plant during root colonization.
Materias Unesco
31 Ciencias Agrarias
Palabras Clave
Biocontrol fungi
Chitin
Chitosan
Aminopolysaccharides
Raman spectroscopy
ISSN
1386-1425
Revisión por pares
SI
Patrocinador
MCIN/AEI/10.13039/501100011033 y el programa NextGenerationEU/PRTR de la UE (PLEC2021-007705)
Junta de Castilla y León y el programa FEDER de la UE (CLU-2019-04 y VA210P20)
Ministerio de Ciencia e Innovación de España (project PID-2020-119734RB-I00)
Junta de Castilla y León y el programa FEDER de la UE (CLU-2019-04 y VA210P20)
Ministerio de Ciencia e Innovación de España (project PID-2020-119734RB-I00)
Version del Editor
Propietario de los Derechos
© 2025 The Author(s)
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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