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2015-03-26T09:14:22Z urn:hdl:10324/10117 CD229 (Ly9) lymphocyte cell surface receptor Interacts homophilically through Its N-Terminal domain and relocalizes to the immunological synapse Romero, Xavier Zapater, Nuria Calvo, María Kalko, Susana G. Fuente García, Miguel Ángel de la Tovar, Victoria Ockeloen, Charlotte Pizcueta, Pilar Engel, Pablo Inmunología Producción Científica CD229 is a member of the CD150 family of the Ig superfamily expressed on T and B cells. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. The cytoplasmic tail of CD229 binds to SAP, a protein that is defective in X-linked lymphoproliferative syndrome. To identify the CD229 ligand, we generated a soluble Ig fusion protein containing the two N-terminal extracellular domains of human CD229 (CD229-Ig). CD229-Ig bound to CD229-transfected cells, whereas no binding was detected on cells expressing other CD150 family receptors, showing that CD229 binds homophilically. Both human and mouse CD229 interacted with itself. Domain deletion mutants showed that the N-terminal Ig-domain mediates homophilic adhesion. CD229-CD229 binding was severely compromised when the charged amino acids E27 and E29 on the predicted B-C loop and R89 on the F-G loop of the N-terminal domain were mutated to alanine. In contrast, one mutation, R44A, enhanced the homophilic interaction. Confocal microscopy image analysis revealed relocalization of CD229 to the contact area of T and B cells during Ag-dependent immune synapse formation. Thus, CD229 is its own ligand and participates in the immunological synapse. 2015-03-26T09:14:22Z 2015-03-26T09:14:22Z 2005 info:eu-repo/semantics/article The Journal of Immunology, 2005, vol. 174, n. 11. p. 7033–7042. 0022-1767 http://uvadoc.uva.es/handle/10324/10117 10.4049/jimmunol.174.11.7033 7033 11 7042 The Journal of Immunology 174 eng https://www.jimmunol.org/content/174/11/7033 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International American Association of Immunologists