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oai:uvadoc.uva.es:10324/101522022-06-28T07:30:28Zcom_10324_1133com_10324_931com_10324_894col_10324_1209

CD84 Functions as a homophilic adhesion molecule and enhances IFN- g secretion:adhesion is mediated by Ig-like domain 1 Martín, Margarita Romero, Xavier Fuente García, Miguel Ángel de la Tovar, Victoria Zapater, Nuria Esplugues, Enric Pizcueta, Pilar Bosch, Jaime Engel, Pablo Biología molecular Producción Científica CD84 is a member of the CD2 subset of the Ig superfamily of cell surface molecules. Its cytoplasmic tail binds to Src homology 2 domain-containing protein 1A (signaling lymphocytic activation molecule-associated protein), a protein encoded by the X-linked lymphoproliferative disease gene. It is preferentially expressed on B lymphocytes, monocytes, and platelets. We show that it is also expressed on thymocytes and T cells. CD84 was positive on CD4 CD8 thymocytes, and its expression decreased with cell maturation. It is expressed on mature T cells preferentially on CD45RO . To identify the CD84 ligand, we generated a soluble Ig fusion protein containing the human CD84 extracellular domains (CD84-Ig). Because receptor-ligand interactions occur between several members of this subfamily, we assayed CD84-Ig binding with all members of the CD2 family. CD84-Ig bound to CD84-transfected cells, whereas no binding was detected with cells expressing other CD2 subfamily receptors, showing that CD84 binds to itself. Anti-CD84 mAbs recognizing epitopes wholly within domain 1 of CD84 blocked the binding of the CD84-Ig fusion protein to CD84-transfected cells and platelets. Data from CD84 domain human/mouse chimeras further revealed that only the first extracellular domain of the molecule is involved in the ligand receptor recognition. The CD84-CD84 interaction was independent of its cytoplasmic tail. Finally, concurrent ligation of human CD84 with mAbs or CD84-Ig and CD3 enhanced IFN-secretion in human lymphocytes. Thus, CD84 is its own ligand and acts as a costimulatory molecule. 2015-04-08T11:33:45Z 2015-04-08T11:33:45Z 2001 info:eu-repo/semantics/article The Journal of Immunology 2001, 167(7): 3668–3676. 0022-1767 http://uvadoc.uva.es/handle/10324/10152 10.4049/jimmunol.167.7.3668 3668 7 3676 The Journal of Immunology 167 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International American Association of Immunologists SI