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oai:uvadoc.uva.es:10324/103602021-06-23T09:48:54Zcom_10324_1133com_10324_931com_10324_894col_10324_1209
UVaDOC author Fuente García, Miguel Ángel de la author Tovar, Victoria author Villamor, Neus author Zapater, Nuria author Pizcueta, Pilar author Campo, Elias author Jaime Bosch, Jaime author Engel, Pablo 2015-04-09T07:45:18Z 2015-04-09T07:45:18Z 2001 Blood, 2001, 97(11):3513-3520 0006-4971 http://uvadoc.uva.es/handle/10324/10360 10.1182/blood.V97.11.3513 3513 11 3520 Blood 97 .Ly-9 is a mouse cell-surface glycoprotein that is selectively expressed on thymocytes and on mature T and B lymphocytes. Ly-9 belongs to the CD2 subset of the immunoglobulin superfamily, an emerging family of cell signaling receptors. Recently, a partial human Ly-9 complementary DNA (cDNA) sequence has been described. Full-length cDNA clones were isolated that included the initiation codon, the sequence encoding the full signal peptide, and 14 amino acids more in the cytoplasmic domain than in the previously reported clone. The predicted extracellular domain of human Ly-9 contains 4 immunoglobulinlike domains, similar to those in mouse Ly-9. Northern blot analysis revealed that the human Ly-9 messenger RNA (2.6 kb) is expressed predominantly in lymph node, spleen, thymus, and peripheral blood leukocytes. Four monoclonal antibodies (mAbs) were raised against human Ly-9 by immunizing mice with the pre-B-cell line 300.19 stably transfected with human Ly-9 full-length cDNA. These mAbs strongly stained the surfaces of cells transfected with human Ly-9 cDNA but not of untransfected cells. Human Ly-9 expression was restricted to T and B lymphocytes and thymocytes, with the highest levels of expression on CD4(+)CD8(-) and CD4(-)CD8(+) thymocytes. Monocytes, granulocytes, platelets, and red blood cells were uniformly negative for Ly-9. These mAbs immunoprecipitated major polypeptides of 120 kd from the transfected cells and 120 kd and 100 kd from B-cell line Daudi, probably because of the cell-surface-expressed isoforms. These data demonstrate that human Ly-9 is a new marker for the study of normal and malignant leukocytes eng Attribution-NonCommercial-NoDerivatives 4.0 International Hematología Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9 info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/10360/1/18.%20M.A.%20de%20la%20Fuente%20PD-231.pdf File MD5 c63f52e5efec67f8572bef841f2de5e0 378232 application/pdf 18. M.A. de la Fuente PD-231.pdf