2026-05-05T22:08:17Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/167382021-06-23T09:52:07Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

López Acosta, José Francisco Villa Pérez, Pablo Fernández Díaz, Cristina María Luis Román, Daniel Antonio de Díaz Marrero, Ana R. Cueto, Mercedes Perdomo Hernández, Germán Cózar Castellano, Irene Diabetes - Tratamiento Producción Científica Diabetes is a consequence of a decrease on functional β-cell mass. We have recently demonstrated that epoxypukalide (Epoxy) is a natural compound with beneficial effects on primary cultures of rat islets. In this study, we extend our previous investigations to test the hypothesis that Epoxy protects β-cells and improves glucose metabolism in STZ-induced diabetic mice. We used 3-months old male mice that were treated with Epoxy at 200 μg/kg body weight. Glucose intolerance was induced by multiple intraperitoneal low-doses of streptozotocin (STZ) on 5 consecutive days. Glucose homeostasis was evaluated measuring plasma insulin levels and glucose tolerance. Histomorphometry was used to quantify the number of pancreatic β-cells per islet. β-cell proliferation was assessed by BrdU incorporation, and apoptosis by TUNEL staining. Epoxy treatment significantly improved glucose tolerance and plasma insulin levels. These metabolic changes were associated with increased β-cell numbers, as a result of a two-fold increase in β-cell proliferation and a 50% decrease in β-cell death. Our results demonstrate that Epoxy improves whole-body glucose homeostasis by preventing pancreatic β-cell death due to STZ-induced toxicity in STZ-treated mice 2016-04-11 2016-04-11 2015 info:eu-repo/semantics/article Islets,(2015);7(2):e1078053 1938-2014 http://uvadoc.uva.es/handle/10324/16738 10.1080/19382014.2015.1078053 e1078053 2 Islets 7 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International Taylor & Francis