2026-04-28T19:19:26Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/220852025-02-19T08:14:30Zcom_10324_1133com_10324_931com_10324_894col_10324_1209

Ibares Frías, Lucía Gallego Muñoz, Patricia Cantalapiedra Rodríguez, Roberto Valsero Blanco, María Cruz Mar Sardaña, Santiago Merayo Lloves, Jesús Martínez García, María del Carmen Gallinas - Ensayos técnicos Córnea - Cirugía Producción Científica Purpose To evaluate corneal wound healing in the hen animal model after additive surgery with an intracorneal ring segment (ICRS). Methods We implanted one ICRS in each eye of 76 hens. In control group 1 (n=22 hens), the stromal channel was prepared but no ICRS was inserted. In control group 2 (n=2 hens), no surgery was performed. Animals were randomly separated into groups and euthanized after clinical follow-up of 4 and 12 hours, 1, 2, 3, and 7 days, and 1, 2, 3, 4, and 6 months. Corneas were stained with hematoxylin-eosin. Apoptosis was measured by terminal uridine nick end-labeling assays. Cell proliferation and myofibroblast-like differentiation were assayed by BrdU and α-smooth muscle actin immunofluorescence microscopy. Stromal matrix changes were documented by electron microscopy. Results Epithelial and stromal cell apoptosis around the ICRS-implanted and control group 1 eyes peaked at 12 hours, but continued for 72 hours. In ICRSimplanted eyes, epithelial and stromal proliferation was present at 12 and 24 hours, respectively, and peaked at 7 days and 72 hours, respectively. Some proliferation in the ICRS-implanted group continued through the 6- month follow-up, and myofibroblast-like cells differentiated one to three months after ICRS implantation. The segments rotated within the stroma as the limbal inferior angle approached the epithelium. Conclusions Wound healing after ICRS implantation in hen corneas was similar to that of other corneal surgical wounds in stages. However, there were some specific features related to the small size of the epithelial wound and the device permanently implanted inside the cornea. 2017-01-12 2017-01-12 2015 info:eu-repo/semantics/article Graefe's Archive for Clinical and Experimental Ophthalmology, (2015); 253:1071–1083 0721-832X http://uvadoc.uva.es/handle/10324/22085 10.1007/s00417-015-2959-5 1071 1083 Graefe's Archive for Clinical and Experimental Ophthalmology 253 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International Springer Verlag