2026-05-05T20:43:43Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/247582021-06-24T07:26:51Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Staubli, Sebastian Manuel Cerino, Giulia González de Torre, Israel Alonso Rodrigo, Matilde Oertli, Daniel Eckstein, Friedrich Glatz, Katharina Rodríguez Cabello, José Carlos Marsano, Anna Producción Científica The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells. Human SVF cells, embedded in RGD-REDV-bioactivated or unmodified ELR-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusion-based culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized SVF-based hydrogels up to 28 days. ELR-based hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells. 2017-07-27 2017-07-27 2017 info:eu-repo/semantics/article Biomaterials Volume 135, August 2017, Pages 30-41 http://uvadoc.uva.es/handle/10324/24758 10.1016/j.biomaterials.2017.04.047 eng http://www.sciencedirect.com/science/article/pii/S0142961217302843 info:eu-repo/grantAgreement/EC/H2020/642687 info:eu-repo/grantAgreement/EC/FP//278557 info:eu-repo/grantAgreement/EC/H2020/646075 info:eu-repo/grantAgreement/EC/FP7/317304 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier