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oai:uvadoc.uva.es:10324/325482025-03-26T19:45:06Zcom_10324_32522com_10324_952com_10324_894col_10324_32524

A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake Alonso Alonso, María Teresa Navas Navarro, Paloma García-Sancho Martín, Francisco Javier Mitocondrias Calcio Mitochondrial Calcium Producción Científica Mitochondrial Ca2+ homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca2+. Ca2+ concentration in mitochondria ([Ca2+]M) is low at rest (about 10−7 M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca2+ uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca2+ transport. Ministerio de Economía, Industria y Competitividad (Project BFU2014-534698P) Instituto de Salud Carlos III (RD16/0011/0003) 2018-11-08T13:03:23Z 2018-11-08T13:03:23Z 2017 info:eu-repo/semantics/bookPart Mokranjac Dejana, Perocchi Fabiana (eds). Mitochondria: Practical Protocols. Humana Press, New York, NY, 2017. Methods in Molecular Biology book series (MIMB, 1567) 978-1-4939-6824-4 http://uvadoc.uva.es/handle/10324/32548 10.1007/978-1-4939-6824-4_15 Mitochondria: Practical Protocols eng Methods in Molecular Biology book series (MIMB, 1567) https://link.springer.com/protocol/10.1007/978-1-4939-6824-4_15 info:eu-repo/semantics/restrictedAccess © Springer Science+Business Media LLC 2017 application/pdf Humana Press https://uvadoc.uva.es/bitstream/10324/32548/3/Introduction-AMicroplate-BasedBioluminescen.pdf.jpg Hispana TEXT http://rightsstatements.org/vocab/CNE/1.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/32548