2026-04-27T21:22:35Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/353302025-03-26T19:10:03Zcom_10324_22821com_10324_954com_10324_894col_10324_22822

Flora, Tatjana González de Torre, Israel Quintanilla Sierra, Luis Alonso Rodrigo, Matilde Rodríguez Cabello, José Carlos 2019-04-03T12:30:55Z 2018 European Polymer Journal, 2018, vol. 106. p. 19-29 0014-3057 http://uvadoc.uva.es/handle/10324/35330 10.1016/j.eurpolymj.2018.06.026 Producción Científica A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out. After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof. Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties. 2020-09 2020-09 Comisión Europea (NMP-2014-646075, MSCA-ITN-2014-642687) Ministerio de Economía, Industria y Competitividad (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R and MAT2016-79435-R) Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. Project VA015U16) Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León application/pdf eng Elsevier info:eu-repo/semantics/openAccess © 2019 Elsevier Control espacial Spatial control Adhesión celular Cell adhesion Biofuncionalización superficial Surface bio-functionalization Ablación láser Laser ablation Recombinantes tipo elastina Elastin-like recombinamers Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers info:eu-repo/semantics/article https://www.sciencedirect.com/science/article/pii/S0014305717322061 SI